Main description:

NMR is one of the most powerful methods for imaging of biomolecules. This book is the ultimate NMR guide for researchers in the biomedical community and gives not only background and practical tips but also a forward looking view on the future of NMR in systems biology.

Contents:

Website: www.wiley-vch.de/home/nmrbiomol INTRODUCTION NMR and its place in Mechanistic Systems Biology (Bertini, McGreevy, Parigi) The structure of biomolecules: fundamentals Structural features of proteins (Banci, Cantini) Structural features of nucleic acids (Cevec, Jonker, Nozinovic, Richter, Schwalbe) What can be learned about the structure and dynamics of biomolecules from NMR Proteins studied by NMR (Ferella, Rosato, Turano) Nucleic acids studied by NMR (Plavec) THE ROLE OF NMR IN THE STUDY OF THE STRUCTURE AND DYNAMICS OF BIOMOLECULES Determination of protein structure and dynamics (Ferella, Rosato, Turano) DNA (Plavec) RNA (Stefl, Sklenar) Intrinsically disordered proteins (Felli, Pierattelli, Tompa) Paramagnetic molecules (Bertini, Luchinat, Parigi) THE ROLE OF NMR IN THE STUDY OF THE STRUCTURE AND DYNAMICS OF BIOMOLECULAR INTERACTIONS NMR methodologies for the analysis of protein interactions (Madl, Sattler) Metal-mediated interactions (Ciofi-Baffoni) Protein-paramagnetic protein interactions (Keizers, Hiruma, Ubbink) Protein-RNA interactions (Manoharan, Perez-Canadillas, Ramos) Protein-DNA interactions (Kovacic, Boelens) NMR IN DRUG DISCOVERY High-throughput screening and fragment-based design: general considerations for lead discovery and optimization (Pellecchia) Ligand-observed NMR in fragment-based approaches (Sledz, Abell, Ciulli) Interactions of metallodrugs with DNA (Sadler) RNA as a drug target (Ferner, Duchardt-Ferner, Rinnenthal, Buck, Wohnert, Schwalbe) Fluorine NMR spectroscopy for biochemical screening in drug discovery (Dalvit) NMR of peptides (Beck, Frank, Kessler) SOLID-STATE NMR Biomolecular solid state NMR - basics (Barbet-Massin, Pintacuda) Protein dynamics in the solid-state (Lewandowski, Emsley) Microcrystalline proteins - An ideal benchmark for methodology development (Franks, van Rossum, Bardiaux, Ravera, Parigi, Luchinat, Oschkinat) Structural studies of protein fibrils by solid-state NMR (Bockmann, Meier) Membrane proteins (Cukkemane, Renault, Baldus) FRONTIERS IN NMR SPECTROSCOPY Dynamic nuclear polarization (Prisner) 13C direct detection NMR (Felli, Pierattelli) Speeding up multidimensional NMR data acquisition (Brutscher, Marion, Frydman) Metabolomics (Tenori) In cell NMR spectroscopy (Burz, Cowburn, Dutta, Shekhtman) Structural investigation of cell-free expressed membrane proteins (Sobhanifar, Reckel, Lohr, Bernhard, Dotsch) COMPUTATIONAL ASPECTS Grid computing (Rosato) Protein-protein docking with HADDOCK (Schmitz, Melquiond, de Vries, Karaca, van Dijk, Kastritis, Bonvin) Automated protein structure determination methods (Guerry, Herrmann) NMR structure determination of protein-ligand complexes (Schieborr, Sreeramulu, Schwalbe) SAXS/SANS as methods complementary to NMR (Petoukhov, Svergun)