Main description:

This volume provides relevant synthetic strategies, incorporation, and applications of non-natural nucleic acids. Chapters detail monomer synthesis, oligomer synthesis/construction, and applications allowing researchers to explore and determine which methodology or methodologies are relevant to their needs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Non-Natural Nucleic Acids: Methods and Protocols aims to serve as a guide for researchers exploring their own inquiries and to provide a springboard for new endeavors.

Contents:

1. Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

Stella Diafa, Damien Evequoz, Christian J. Leumann, and Marcel Hollenstein

2. Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 2'-Deoxycytidine

Na An, Aaron M. Fleming, Nicole C. Rosecrans, Yi Liao, and Cynthia J. Burrows

3. Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue

Hui Mei and John Chaput

4. Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA

Petra Menova, Hana Cahova, Milan Vrabel, and Michal Hocek

5. 2'-C,4'-C-Ethyleneoxy-Bridged 2'-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability

Takashi Osawa, Satoshi Obika, and Yoshiyuki Hari

6. Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids

Tanaya Bose and Vaijayanti A. Kumar

7. Synthesis of Nucleobase-Functionalized Morpholino Monomers

Bappaditya Nandi, Sankha Pattanayak, Sibasish Paul, Jayanta Kundu, and Surajit Sinha

8. Synthesis and Application of LK T Peptide Nucleic Acids

Nathaniel Shank, Kara M. George Rosenker, Ethan A. Englund, Andrew V. Dix, Elizabeth E. Rastede, and Daniel H. Appella

9. Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids

Derrick Watkins, Krishnagopal Maiti, and Dev P. Arya

10. Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site

Maryam Imani Nejad, Xu Guo, Kurt Housh, Christopher Nel, Zhiyu Yang, Nathan E. Price, Yinsheng Wang, and Kent S. Gates

11. Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids

Malte Rosenthal, Franziska Pfeiffer, and Gunter Mayer

12. Labeling Peptide Nucleic Acids with Indium-111

Igor G. Panyutin

13. Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet

Michael P. Ledbetter, Denis A. Malyshev, and Floyd E. Romesberg

14. Flexible Nucleic Acids (FNAs) As Informational Molecules: Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates

Maryline Chemama and Christopher Switzer

15. Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis

Jung-Suk Choi and Anthony Berdis

16. FRET Assay for Ligands Targeting the Bacterial A-Site RNA

Renatus W. Sinkeldam and Yitzhak Tor

17. The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons

Keiji Murayama, Hiromu Kashida, and Hiroyuki Asanuma

18. Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA

Timofei S. Zatsepin, Anna M. Varizhuk, Vladimir G. Dedkov, German A. Shipulin, and Andrey V. Aralov

19. Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation

Hailey L. Gahlon and Shana J. Sturla