Main description:

This detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the book organizes these key methods in order to achieve a unifying comprehension of the olfactory system. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and multidisciplinary, Olfactory Receptors: Methods and Protocols serves to benefit not only those who are beginning in the field of olfactory receptors, but also those who are already working in the field and that want to learn another advanced techniques.

Contents:

1. CD36 Neuronal Identity in the Olfactory Epithelium

Andre Machado Xavier and Isaias Glezer

2. Deorphanization of Olfactory Trace Amine-Associated Receptors

Qian Li

3. G Protein-Coupled Receptor Kinase 3 (GRK3) in Olfaction

Sayoko Ihara and Kazushige Touhara

4. Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcium Imaging

Pablo Chamero and Frank Zufall

5. Calcium Imaging of Individual Olfactory Sensory Neurons from Intact Olfactory Turbinates

Chunbo Zhang

6. Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations

Artur G. Leme Silva, Maira H. Nagai, and Bettina Malnic

7. Numerical Models and In Vitro Assays to Study Odorant Receptors

Caroline Bushdid, Claire A. de March, Hiroaki Matsunami, and Jerome Golebiowski

8. High-Throughput Odorant Receptor Deorphanization Via pS6-Immunoprecipitation and mRNA Profiling

Xiaoyang Serene Hu and Hiroaki Matsunami

9. Patch-Clamp Recordings from Mouse Olfactory Sensory Neurons

Anna Boccaccio

10. In Vivo Electrophysiological Recordings of Olfactory Receptor Neuron Units and Electro-Olfactograms in Anesthetized Rats

Patricia Duchamp-Viret and Michel Chaput

11. Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction

Michele Dibattista and Johannes Reisert

12. Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ

Xavier Grosmaitre and Minghong Ma

13. Long-term Plasticity at the Mitral and Tufted Cell to Granule Cell Synapse of the Olfactory Bulb Investigated with a Custom Multi-Electrode in Acute Brain Slice Preparations

Michael Lukas, Knut Holthoff, and Veronica Egger

14. Multi-Site Recording of Local Field Potentials in Awake, Free-Moving Mice

Xingfeng Mao, Tiantian Cao, and Anan Li

15. In Vivo Two-Photon Imaging of the Olfactory System in Insects

Marco Paoli and Albrecht Haase

16. Approaches for Assessing Olfaction in Children with Autism Spectrum Disorder

Hirokazu Kumazaki, Masako Okamoto, Syo Kanzaki, Ken-ichi Okada, Masaru Mimura, Yoshio Minabe, and Mitsuru Kikuchi

17. Methods in Rodent Chemosensory Cognition

Alan Gelperin

18. Bioelectronic Nose Using Olfactory Receptor-Embedded Nanodiscs

Heehong Yang, Minju Lee, Daesan Kim, Seunghun Hong, and Tai Hyun Park

19. Tracking Odorant Plumes

Matthew A. Reidenbach

20. Generative Biophysical Modeling of Dynamical Networks in the Olfactory System

Guoshi Li and Thomas A. Cleland

21. Behavioral Assays in the Study of Olfaction: A Practical Guide

Fabio Papes, Thiago S. Nakahara, and Antonio P. Camargo