Main description:

This volume provides researchers with protocols that help them investigate known or putative O2 sensing proteins and pathways. The chapters in this book discuss techniques ranging from anaerobic redox midpoint measurement to approaches to control expression of globin genes, which provide detailed methods for researchers interested in expanding their knowledge of O2 sensing systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Oxygen Sensing: Methods and Protocols is a valuable resource to a wide audience, ranging from microbiologists and cell biologists, to protein biochemists.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Integrating UV-Vis Spectroscopy and Oxygen Optode for Accurate Determination of Oxygen Affinity of Proteins
Anoop Rama Damodaran and Ambika Bhagi-Damodaran

2. Measurement of O2 Binding by Sensory Hemeproteins
Marie A. Gilles-Gonzalez and Eduardo H. S. Sousa

3. Resonance Raman Characterization of O2-Binding Heme Proteins
Samuel N. Snyder, Tapiwa Chiura, and Piotr J. Mak

4. Anaerobic Infra-Red Spectroelectrochemical Methods for Studying Oxygen-Sensitive [FeFe] Hydrogenases
Patrick Corrigan and Alexey Silakov

5. Monitoring the Kinase Activity of Heme-Based Oxygen Sensors and Its Dependence on O2 and Other Ligands using Phos-tag Electrophoresis
Jakub Vavra, Artur Sergunin, Toru Shimizu, and Marketa Martinkova

6. In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity
Nushrat J. Hoque, Madison P. Helm, and Emily E. Weinert

7. Spectrophotometric Method for the Quantification and Kinetic Evaluation of In Vitro c-di-GMP Hydrolysis in the Presence and Absence of Oxygen
Dayna C. Patterson and Emily E. Weinert

8. Hydrogen/Deuterium Exchange Mass Spectrometry of Heme-Based Oxygen Sensor Proteins
Jakub Vavra, Artur Sergunin, Martin Stranava, Alan Kadek, Toru Shimizu, Petr Man, and Marketa Martinkova

9. Methods for Biophysical Characterization of SznF, A Member of the Heme-Oxygenase-Like Diiron Oxidase/Oxygenase Superfamily
Molly J. McBride, Sarah M. Pope, Mrutyunjay A. Nair, Debangsu Sil, Xavier E. Salas-Sola, Carsten Krebs, J. Martin Bollinger Jr., and Amie K. Boal

10. Analyzing Iron and Oxygen-Regulated Protein Complex Formation using Proteomic Mass Spectrometry
Vijaya Pandey, Adarsh K. Mayank, and James A. Wohlschlegel

11. CRISPR Activator Approaches to Study Endogenous Androglobin Gene Regulation
Teng Wei Koay, Johannes Schoedel, and David Hoogewijs

12. Assays to Study Hypoxia-Inducible Factor Prolyl Hydroxylase Domain 2 (PHD2), A Key Human Oxygen Sensing Protein
Yan Ying Chan, Naasson M. Mbenza, Mun Chaing Chan, and Ivanhoe K. H. Leung

13. Kinetic Measurement Investigating the Oxygen-Sensing Properties of the Plant Cysteine Oxidases
Anna Dirr, Dona M. Gunawardana, and Emily Flashman

14. Methods for Culturing Anaerobic Microorganisms
Michel Geovanni Santiago-Martinez, and James Gregory Ferry

Subject Index List...