Main description:

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.

Contents:

1. An Introduction to Patch Clamp Recording

Charlotte L. Hill and Gary J. Stephens

2.Patch Clamp Technology in the 21st Century

Jan Dolzer

3. Heterologous Expression of Ion Channels in Mammalian Cell Lines

Alistair Mathie, Emma L. Veale, and Robyn G. Holden

4. Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel

Ekaterina Zaitseva, Alison Obergrussberger, Conrad Weichbrodt, Mordjane Boukhet, Frank Bernhard, Christopher Hein, Gerhard Baaken, Niels Fertig and Jan C. Behrends

5. Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology

Kadla R. Rosholm, Kim Boddum, and Anders Lindquist

6. Multi-electrode Arrays

Russell Burley and Jenna R M Harvey

7. Utilising Automated Electrophysiological Platforms in Epilepsy Research

Carol J. Milligan and Svenja Pachernegg

8. Dynamic Clamp on a Windows PC

Lorin S. Milescu

9. Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing Kashif Mahfooz and Tommas J. Ellender

10. Bioluminescence Methodology for Ion Channel Studies

Paul A Wadswort,, Aditya K. Singh, Nghi Nguyen, Clifford Stephan, and Fernanda Laezza

11. Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons

Francesco Tamagnini

12. Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method

Timothy W. Church and Matthew G. Gold

13. In Vivo Patch Clamp Studies

Yi Zhou, He Li and Zhongju Xiao

14. In Vivo Optogenetics with Stimulus Calibration

Luke T. Coddington and Joshua T. Dudman

15. Single or Double Patch Clamp Recordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics

Jean Simonnet, Louis Richevaux, and Desdemona Fricker

16. Optogenetics and Optical Tools in Automated Patch Clamping

Kim Boddu, Peder Skafte-Pedersen, Jean-Francois Rolland, and Sandra Wilson