Main description:

This volume discusses a variety of preclinical MRI methods and protocols to help technicians and researchers conduct studies in their respective fields. This book is organized into 7 parts: Part 1 covers the basics of MRI physics, relaxation, image contrast, and main acquisition sequences; Part 2 describes methodologies for diffusion, perfusion, and functional imaging; Part 3 looks at in vivo spectroscopy; Part 4 explores special MRI techniques that are less known in the field; Parts 5 and 6 discuss MRIs and MRSs in animal models of disease and the applications used to study them, and Part 7 looks at anesthesia and advanced contrast agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Preclinical MRI: Methods and Protocols is a valuable resource for researchers interested in expanding their knowledge in this developing field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

PART I: MRI BASICS
1. Introduction to MRI Physics
Gary Martinez

2. Basic Pulse Sequences in Magnetic Resonance Imaging
Daniel Calle and Teresa Navarro

PART II: PERFUSION, DIFFUSION AND FUNCTIONAL MRI
3. Dynamic Susceptibility Contrast MRI in Small Animals
Pilar Lopez-Larrubia

4. Preclinical Arterial Spin Labeling Measurement of Cerebral Blood Flow
Eric R. Muir

5. Dynamic Contrast-Enhanced MRI
Jennifer Moroz and Stefan A. Reinsberg

6. Diffusion Weighted Magnetic Resonance Imaging
Irene Guadilla, Daniel Calle, and Pilar Lopez-Larrubia

7. Diffusion Tensor Imaging (DTI)
Silvia Lope-Piedrafita

8. Mapping Functional Connectivity in the Rodent Brain using Electric-Stimulation fMRI
Laura Perez-Cervera, Jose Maria Carames, Luis Miguel Fernandez-Molla, Andrea Moreno, Begona Fernandez, Elena Perez-Montoyo, David Moratal, Santiago Canals, Jesus Pacheco-Torres

9. Functional Diffusion Magnetic Resonance Imaging
Rita Maria Rocha Oliveira, Irene Guadilla, and Pilar Lopez-Larrubia

PART III: IN VIVO SPECTROSCOPY

10. In Vivo 1H Magnetic Resonance Spectroscopy
M. Carmen Munoz-Hernandez and Maria L. Garcia-Martin

11. In Vivo Heteronuclear Magnetic Resonance Spectroscopy
Blanca Lizarbe, Antoine Cherix, and Rolf Gruetter

12. 1H Spectroscopic Imaging of the Rodent Brain
Rui V. Simoees, Emma Munoz-Moreno, Raul Tudela, and Guadalupe Soria

PART IV: SPECIAL MRI TECHNIQUES

13. Susceptibility Weighted MRI in Rodents at 9.4T
Ferdinand Schweser, Marilena Preda, and Robert Zivadinov

14. Biomedical 19F MRI Using Perfluorocarbons
Tuba Guden-Silber, Sebastian Temme, Christoph Jacoby, and Ulrich Floegel

15. Rodent Abdominal Adipose Tissue Imaging by MR
Bhanu Prakash KN, Jadegoud Yaligar, Sanjay K. Verma, Venkatesh Gopalan, Sendhil Velan S.

16. Cardiac MRI in Small Animals
Min-Chi Ku, Till Huelnhagen, Thoralf Niendorf, and Andreas Pohlmann

17. In Utero MRI in Mouse Embryos
Jiangyang Zhang, Dan Wu, and Daniel H. Turnbull

18. Oxygenation Imaging by Nuclear Magnetic Resonance Methods
Heling Zhou, Nuria Arias-Ramos, Pilar Lopez-Larrubia, Ralph P. Mason, Sebastian Cerdan, and Jesus Pacheco-Torres

19. Molecular Magnetic Resonance Imaging (mMRI)
Maxime Gauberti, Antoine Fournier, Denis Vivien, and Sara Martinez De Lizarrondo

PART V: MRI AND MRS IN ANIMAL MODELS OF DISEASE

20. Magnetic Resonance Spectroscopy Studies of Mouse Models of Cancer
Menglin Cheng and Kristine Glunde

21. MRI in the Study of Animal Models of Neurodegenerative Diseases
Nyoman D. Kurniawan

22. MRI in the Study of Animal Models of Stroke
Pedro Ramos-Cabrer and Daniel Padro

PART VI: OTHER APPLICATIONS

23. Assessment of Blood Brain Barrier Leakage with Gadolinium Enhanced MRI
Min-Chi Ku, Sonia Waiczies, Thoralf Niendorf, and Andreas Pohlmann

24. In Vivo Pharmacokinetics of Magnetic Nanoparticles
Carlos Caro, M. Carmen Munoz-Hernandez, Manuel Pernia Leal, and Maria L. Garcia-Martin

PART VII: ANESTHESIA AND ADVANCED CONTRAST AGENTS

25. Anaesthesia and Monitoring of Animals during MRI Studies
Jordi L. Tremoleda, Sven Macholl, and Jane K. Sobawoski

26. Advanced Contrast Agents for Multimodal Biomedical Imaging Based in Nanotechnology
Daniel Calle, Paloma Ballesteros, and Sebastian Cerdan