Main description:

The only resource to systematically review current experimental methods, this handy reference enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. After an initial section on probing the lipid bilayer, the text moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. The first single publication to incorporate chemical markers, fluorescent probes and genetic tags allows a well-informed comparison of different solutions for the same problem in molecular analytics.

Contents:

Fluorescent Sterols to Study Cholesterol Trafficking in Living Cells Lipid Binding Proteins to Study Localization of Phosphoinositides The Use of Lipid-Binding Toxins to Study Distribution and Dynamics of Sphingolipids and Cholesterol FlAsH Protein Labeling AGT/SNAP-tag: A Versatile Tag for Covalent Protein Labeling In Vivo Protein Labelling with Trimthoprim and Dihydrofolate Reductase Phosphopantetheinyl Transferase Catalyzed Protein Labeling and Molecular Imaging Bioorthogonal Chemical Transformations in Proteins by an Expanded Genetic Code Using the Bacteriophage MS2 Coat Protein-RNA Binding Interaction to Visualise RNA in Living Cells