Main description:

This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.

Contents:

Series Preface...

Preface...

Table of Contents...

Contributing Authors...

Part I Introduction

1. Introduction to Synaptosome Methods and Applications

Kathryn M. Murphy

Part II Introduction to Synaptosomes

2. Victor P. Whittaker: The Discovery of the Synaptosome and Its Implications

Herbert Zimmermann

3. Synaptosome Preparations: Which Procedure Should I Use?

Peter R. Dunkley and Phillip J. Robinson

Part III Making Synaptosomes

4. Protocol for a High-Throughput Semi-Automated Preparation for Filtered Synaptoneurosomes

Justin L. Balsor and Kathryn M. Murphy

5. Subcellular Fractionation of Brain Tissue from Small Tissue Explants

Veronique De-Smedt-Peyrusse, Laetitia Darriet, Pierre Trifilieff, Etienne Herzog, and Maria Florencia Angelo

6. Preparation of P2 of Percoll-Purified Synaptosomes from Mammalian Brain Tissue

Jing Xue, Annie Quan, and Phillip J. Robinson

Part IV Studying Synaptic Energy and Metabolism using Synaptosomes

7. Synaptosomal Bioenergetics and Glutamate Release

David G. Nicholls

8. Synaptosomes Bioenergetics and Calcium Handling: Aging Response

Silvia Lores-Arnaiz, Georgina Rodriguez de Lores Arnaiz, Analia G. Karadayian, and Juanita Bustamante

9. Isolation of Highly Purified and Functionally Active Synaptic Mitochondria

Sergei V. Baranov, Nicolas K. Khattar, Svitlana Yablonska, Oxana V. Baranova, Eric S. Kretz, Timothy M. Larkin, Diane L. Carlisle, and Robert M. Freidlander

10. Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes

Kasper Engholm-Keller, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson, and Mark E. Graham

Part V Studying Synaptic Proteomics, Function, and Plasticity using Synaptosomes

11. Manipulating Synapse-Enriched Biochemical Fractions to Detect a Purified Synaptic Protein's Effect on Neurotransmitter Release

Marianne Cilluffo, Theodore A. Sarafian, and Joseph B. Watson

12. Prosomes: Supramolecular Structures at the Synaptosome Membrane Involved in Vesicle Docking, Fusion, and Neurotransmitter Release

Bhanu P. Jena

13. SMPL-Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Cell Biology of Synapses

Riana Lo Bu, Jeffrey Clark, Thomas DiChiara, Stephen G. Sligar, and William L. Klein

Part VI Studying Development and Disease using Synaptosomes

14. Profiling of MicroRNA and Protein from Purified Synaptoneurosomes in a Neurodegenerative Disease Model

Amrit S. Boese, Anna Majer, and Stephanie A. Booth

15. Use of Synaptoneurosome Samples to Study Development and Plasticity of Human Cortex

Caitlin R. Siu, Simon P. Beshara, Justin L. Balsor, Steve J. Mancini, and Kathryn M. Murphy

16. The Use of Synaptosomes in Studying Autism Spectrum Disorder and Other Neurodevelopmental Disorders

Nadeem Murtaza, Vickie Kwan, Biren Dave, and Karun K. Singh

17. Flow Cytometric Evaluation of Crude Synaptosome Preparation as a Way to Study Synaptic Alteration in Neurodegenerative Diseases

Nadia P. Postupna, Caitlin S. Latimer, C. Dirk Keene, Kathleen S. Montine, Thomas J. Montine, and Martin Darvas