Main description:

This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strategies to assist in nanocarrier targeting to specific cells and tissues; and methods for evaluating delivery and efficiency for new classes of agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Targeted Drug Delivery: Methods and Protocols is a valuable resource for anyone interested in understanding the diversity of methods used in this evolving field.

Contents:

Preface...Table of Contents...Contributing Authors...
Part I Drug Carrier Design
1. Synthesis of Cationic Polymer Libraries for Gene Delivery using Diglycidyl EthersJacob Elmer, Thrimoorthy Potta, and Kaushal Rege
2. Generation of Ultra-Small PLGA Nanoparticles by Sequential CentrifugationXingwang Wu, Jiangbing Zhou, and Toral R. Patel
3. Construction of Bacteria-Based Cargo Carriers for Targeted Cancer TherapyMahama A. Traore, Ali Sahari, and Bahareh Behkam
4. Production of Extracellular Vesicles Loaded with Therapeutic CargoTek N. Lamichhane and Steven M. Jay
5. Delivery of Cytotoxic Mesenchymal Stem Cells with Biodegradable Scaffolds for Treatment of Postoperative Brain CancerKevin T. Sheets, Juli R. Bago, and Shawn D. Hingtgen
6. Nanoparticles for Targeted Drug Delivery to Cancer Stem-Like Cells and TumorHai Wang and Xiaoming He
Part II Passive and Active Targeting Methods
7. Exploiting Phage Display for Development of Novel Cellular Targeting StrategiesWilliam Marsh, Amanda Witten, and Sarah E. Stabenfeldt
8. CD44 Targeted Lipid Nanoparticles for MicroRNA TherapyStephen L. Hayward and Srivatsan Kidambi
9. Ultrasound and Microbubble-Mediated Blood-Brain Barrier Disruption for Targeted Delivery of Therapeutics to the BrainMeaghan A. O'Reilly and Kullervo Hynynen
10. In Vitro Validation of Targeting and Comparison to Mathematical ModelingJill M. Steinbach-Rankins and Michael R. Caplan
Part III Measuring Delivery and Efficacy
11. Enzyme-Linked Immunosorbent Assay to Quantify Targeting Molecules on NanoparticlesRachel S. Edelstein, Jilian R. Melamed, and Emily S. Day
12. Tunable Collagen Microfluidic Platform to Study Nanoparticle Transport in the Tumor MicroenvironmentMatthew R. DeWitt and M. Nichole Rylander
13. Utilizing the Lung as a Model to Study Nanoparticle Based Drug Delivery SystemsDylan K. McDaniel, Veronica M. Ringel-Scaia, Sheryl L. Coutermarsh-Ott, and Irving C. Allen
14. Non-Enzymatic Tissue Homogenization for Biodistribution AnalysisDanielle M. DiPerna, Alesia V. Prakapenka, Eugene P. Chung, and Rachael W. Sirianni
15. Methods and Study Designs for Characterizing the Pharmacokinetics and Pharmacodynamics of Carrier-Mediated AgentsAllison N. Schorzman, Andrew T. Lucas, John R. Kagel, and Willliam C. Zamboni