Main description:

This volume explores the latest techniques used to study neurodevelopmental diseases (NDD) that range from molecular aspects to integrated research approaches and brain imaging in living rodents. Chapters in this book cover topics such as protocols to deliver shRNA in vitro and in vivo using lentiviral particles to knock-down specific protein expression; experimental procedures to use recombinant fluorescent probes to visualize endogenous proteins at the mammalian synapse; CRISPR/Cas9 toolkit to assess either gain- or loss-of-gene function in brain organoids; detailed protocols to use in vivo manipulations to correct the behavioral phenotypes in cognitive disorder mouse lines; and experimental approaches to genetically engineer macaque models of NDDs and investigate how genetic predisposition may cause neural and functional alterations. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and practical, Translational Research Methods in Neurodevelopmental Disorders is a valuable resource for all researchers who want to learn more about this important and developing field.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Using Lentiviral shRNA Delivery to Knock-Down Proteins in Cultured Neurons and In Vivo
Kevin A. Wilkinson, Kirsty J. McMillan, Paul J. Banks, Ruth E. Carmichael, Yasuko Nakamura, Zafar I. Bashir, Peter J. Cullen, and Jeremy M. Henley

2. Synaptosomal Preparation and Its Application in Preclinical Studies
Marco Feligioni, Lucia Buccarello, Kambiz Hassanzadeh, and Massimo Corbo

3. Assessing the Nanoscale Organization of Excitatory and Inhibitory Synapses using Recombinant Probes to Visualize Endogenous Synaptic Proteins
Matteo Fossati, Marco Erreni, Martina Biagioni, and Alessandra Folci

4. Agonist-Induced Functional Analysis and Cell Sorting (aiFACS) to Select and Characterize Interneurons during Brain Development
Julie Cazareth, Marielle Jarjat, Frederic Brau, Barbara Bardoni, and Thomas Maurin

5. A CRISPR/Cas9-Based Toolkit to Test Gain- and Loss-of-Gene Function in Brain Organoids
Anne Le Digarcher, Celine Lemmers, Arnaud Monteil, Charles Hong, Annie Varrault, and Tristan Bouschet

6. Assessment of Adult Mouse Brain NeuroAnatomical Phenotypes using Quantitative and Precision Histology
Stephan C. Collins and Binnaz Yalcin

7. Leveraging Microelectrode Array Technology for Phenotyping Stem Cell-Derived Neurodevelopmental Disease Models
Shan Wang, Eline van Hugte, Ummi Ciptasari, and Nael Nadif Kasri

8. Humanized Chimeric Mouse Models to Study Human Neural Development and Pathogenesis of Brain Diseases
Camille Thiberge, Maria Llach Pou, Aline Vitrac, Uwe Maskos, and Isabelle Cloez-Tayarani

9. In Vivo Manipulations to Correct for Behavioral Phenotypes in Cognitive Disorder Mouse Models
Chun-Lei Zhang, Mattia Aime, and Yann Humeau

10. Assessing Dyadic Social Interactions in Rodent Models of Neurodevelopmental Disorders
Sara Schiavi, Antonia Manduca, Emilia Carbone, Valeria Buzzelli, and Viviana Trezza

11. Central and Peripheral Explorations by Metabolomics to Study Neurodevelopmental Disorders
Alexandra Bourdin-Pintueles, Laurent Galineau, Antoine Lefevre, Lydie Nadal-Desbarats, Sylvie Mavel, and Patrick Emond

12. Genetic Engineering of Non-Human Primates Models for Studying Neurodevelopmental Disorders
Rahul Suresh, Platon Megagiannis, Hong Chen, Rogier Landman, Jitendra Sharma, and Yang Zhou

Subject Index List...