Main description:

This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab.
Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels.

Contents:

1. Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels

Pau Donate-Macian, Alba Crespi-Boixader, and Alex Peralvarez-Marin

2. Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6

Appu K. Singh, Luke L. McGoldrick, and Alexander I. Sobolevsky

3. Cryo-Electron Microscopy of TRP Channels

Amrita Samanta, Taylor E.T. Hughes, and Vera Y. Moissenkova-Bell

4. Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation

Veronica I. Dumit, Michael Koettgen, and Alexis Hofherr

5. Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels

Victoria Oakes and Carmen Domene

6. Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique

Elvira de la Pena and Ana Gomis

7. Fluorescence-Based Functional Assays for Ca2+ Permeable ThermoTRP Channels

Celia Cordero-Sanchez, Irene Mudarra-Fraguas, and Asia Fernandez-Carvajal

8. High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons

Joerg Isensee and Tim Hucho

9. Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels

Beiying Liu and Feng Qin

10. TRP Channels Reconstitution in Lipid Bilayers

Eleonora Zakharian

11. Methods for Investigating TRP Channel Gating

Osvaldo Alvarez, Karen Castillo, Emerson Carmona, Carlos Gonzalez, and Ramon Latorre

12. In Silico Approaches for TRP Channel Modulation

Magdalena Nikolaeva Koleva and Gregorio Fernandez-Ballester

13. Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction

Tracey Pirali, Ubaldina Galli, Marta Serafini, Alessia Griglio, Armando A. Genazzani, and Gian Cesare Tron

14. In Vivo Methods to Study ThermoTRP Channels in Rodents

Sara Gonzalez-Rodriguez