Main description:

This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now?John C. Clapham
2. Practical Considerations for In Vivo StudiesEdward T. Wargent
3. Nutritional Models of Type 2 Diabetes MellitusBeverly Sara Muhlhausler, Carla Toop, and Sheridan Gentili
4. Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 DiabetesPaul W. Finn
5. Whole Exome Sequencing (WES) for Illumina HISeq using Solution-Based CaptureMilind C. Mahajan and Andrew S. McLellan
6. Gene Expression Mining in Type 2 Diabetes ResearchDonald R. Dunbar
7. Pathways Enrichment Analysis of Gene Expression Data in Type 2 DiabetesMaysson Ibrahim
8. Diagnostic Genetic Testing for Monogenic Diabetes and Congenital HyperinsulinemiaJayne A.L. Houghton
9. The Isolation and Purification of Rodent Pancreatic Islets of LangerhansJacqueline F. O'Dowd and Claire J. Stocker
10. Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow CytometryMatthew J. Butcher, Michelle B. Trevino, Yumi Imai, and Elena V. Galkina
11. Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin ImmunoprecipitationIonel Sandovici, Lisa Nicholas, and Laura P. O'Neill
12. Quantification of Pancreatic Islets: Using Image Analysis ToolsParvathy E. Harikumar
13. The Measurement of Calcium Signaling In Beta Cell Lines using Epifluorescence and Confocal MicroscopyJoanne L. Selway
14. Nitric Oxide and Redox State Measurements in Pancreatic Beta CellsRodrigo Carlessi, Vinicius Cruzat, Younan Chen, and Philip Newsholme
15. Primary Adipocytes as a Model for Insulin SensitivityMohamed S. Zaibi
16. Assessing Islet Transplantation Outcome in MiceAileen J.F. King and Chloe L. Rackham
17. The Measurement of Insulin Secretion using Pancreas Perfusion in the RodentEdward T. Wargent