Main description:

This volume provides a practical guide providing step-by-step protocol to explore vaccines for farm and companion animals, as well as for fish and insects. Divided into three volumes, Volume 2: Vaccines for Veterinary Diseases guides readers through veterinary vaccines, vaccines for poultry, vaccines for farm animals, and vaccines for veterinary parasites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and practical, Vaccine Design: Methods and Protocols, Second Edition, Volume 2: Vaccines for Veterinary Diseases aims to be a useful practical guide to researchers to help further their study in this field.

Contents:

PART I VETERINARY VACCINES: INTRODUCTION

1. Challenges in Veterinary Vaccine Development

Sunil Thomas, Ann Abraham, Alina Rodriguez-Mallon, Sasimanas Unajak, and John P. Bannantine

PART II TRENDS IN VETERINARY VACCINES

2. Mammalian Cell Culture as a Platform for Veterinary Vaccines

Thailin Lao Gonzalez, Ileanet Avalos Olivera, and Alina Rodriguez-Mallon

3. Avian Paramyxoviruses as Vectors for Vaccine Development

Shin-Hee Kim, Edris Shirvani, and Siba Samal

PART III VACCINES FOR POULTRY

4. Reverse Genetics and its Usage in the Development of Vaccine against Poultry Diseases

Barnali Nath, Sudhir Morla, and Sachin Kumar

PART IV VACCINES FOR FARM ANIMALS

5. Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies Paratuberculosis Vaccines

John P. Bannantine, Tuhina Gupta, Denise K. Zinniel, Ahmed Hikal, Frederick D. Quinn, and Raul G. Barletta

6. Recombinant Escherichia coli Cell lysates as a low-cost alternative for vaccines against veterinary clostridial diseases

Clovis Moreira Jr, Gustavo Marcal Schmidt Garcia Moreira, Rafael Amaral Donassolo, Marcos Roberto Alves Ferreira, Mariliana Luiza Ferreira Alves, Rafael Rodrigues Rodrigues, and Fabricio Rochedo Conceicao

7. Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli

Mariliana Luiza Ferreira Alves, Gustavo Marcal Schmidt Garcia Moreira, Marcos Roberto Alves Ferreira, Rafael Amaral Donassolo1, Clovis Moreira Jr, Rafael Rodrigues Rodrigues, and Fabricio Rochedo Conceicao

PART V VACCINES FOR VETERINARY PARASITES

8. Macrophage Stimulation as a Useful Approach for Immunoscreening of Potential Vaccine Candidates against Toxoplasma gondii and Neospora caninum Infections

Ragab M. Fereig and Yoshifumi Nishikawa

PART VI VACCINES FOR FISH

9. Fundamentals of Fish Vaccination

Megha Kadam Bedekar and Sajal Kole

10. Teleost Fish as an Experimental Model for Vaccine Development

Marco Antonio de Andrade Belo and Ives Charlie-Silva

11. Development of Nano-conjugated DNA Vaccine against Edwardsiellosis Disease in Fish

Megha Kadam Bedekar and Sajal Kole

12. Development and Evaluation of DNA Vaccine against Salmonid Alphavirus

Chia-Jung Chang

13. Novel Vaccine Development for Fish Culture Based on the Multiepitope Concept

Sasimanas Unajak, Ansaya Pumchan, Sittiruk Roytrakul, Orathai Sawatdichaikul,

and Nontawith Areechon

14. Testing Novel Inactivation Methods and Adjuvants for Vaccines against Streptococcus Agalactiae in Nile Tilapia Oreochromis niloticus

Fernando Carlos Ramos-Espinoza, Victor Alexander Cueva-Quiroz, Jefferson Yunis-Aguinaga, Norquis Caled Alvarez-Rubio, Nicoli Paganoti de Mello, and Julieta Rodini Engracia de Moraes

PART VII VACCINES AGAINST TICKS

15. Anti-tick Vaccines: Current Advances and Future Prospects

Dennis Muhanguzi, Christian Ndekezi, Joseph Nkamwesiga, Shewit Kalayou, Sylvester Ochwo, Moses Vuyani, and Magambo Phillip Kimuda

16. Vaccines against Vector-Borne Diseases

Chrysoula Kitsou and Utpal Pal

17. A quantum vaccinomics approach based on protein-protein interactions

Marinela Contreras, Sara Artigas- Jeronimo, Juan J. Pastor Comin, and Jose de la Fuente

18. Screening for the 'Achilles Heel' of Hyalomma anatolicum Ticks by RNA Interference Technology and an Update on anti-Tick Vaccine Design

Manjunathachar HV, Azhahianambi P, Binod Kumar, and Ghosh S

19. Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response

Kodai Kusakisako, Takeshi Miyata, Kozo Fujisaki, and Tetsuya Tanaka

20. Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction

Seham HM Hendawy, Heba F. Alzan, Tetsuya Tanaka, and Mona