Main description:

This second edition includes a collection of antigen production and delivery strategies for vaccine development in veterinary species. New and updated chapters guide readers through protocols for antigen production, experimental antigen delivery and the analysis of immune responses upon vaccination. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols, Second Edition aims to be a useful and practical guide to researches to help further their study in this field.

Contents:

1. An overview of veterinary viral diseases and vaccine technologies

Alejandro Brun

2 .Production and purification of candidate subunit vaccines by IC-tagging protein encapsulation

Natalia Barreiro-Pineiro; Tomas Pose Boirazian; Rebeca Menaya-Varga, and Jose M. Martinez-Costas.

3. Elastin-like polymers as nanovaccines: protein engineering of self-assembled, epitope-exposing nanoparticles

Alessandra Girotti, Juan Gonzalez-Valdivieso, Irene Alonso-Sampedro, Sara Escalera-Anzola, Sandra Ramos-Diez, and F. Javier Arias

4. Display of heterologous proteins in Bacillus subtilis biofilms for enteric immunization

Ramona Wissmann, Cornel Fraefel, Claudio Aguilar, and Catherine Eichwald

5. Production of influenza H5 vaccine oligomers in plants

Hoang Trong Phan and Udo Conrad

Chapter 6

DNA vaccines in pigs: from immunization to antigen identification.

Francesc Accensi, Laia Bosch-Camos, Paula L. Monteagudo and Fernando Rodriguez

7. Use of foot-and-mouth disease virus non-coding synthetic RNAs as vaccine adjuvants

Miguel Rodriguez-Pulido, Miryam Polo, Belen Borrego, and Margarita Saiz

8. Evaluation of innate lymphoid cells and dendritic cells following viral vector vaccination.

Zheyi Li, Sreeja Roy, and Charani Ranasinghe

9. Generation of replication deficient human adenovirus 5 (Ad5) vectored FMD vaccines.

Gisselle N. Medina, Teresa de los Santos, and Fayna Diaz-San Segundo

10. Recombinant modified vaccinia virus Ankara development to express VP2, NS1, and VP7 proteins of Bluetongue virus

Alejandro Marin-Lopez, Sergio Utrilla-Trigo, Luis Jimenez-Cabello, and Javier Ortego.

11. Cloning strategies for the generation of recombinant capripoxvirus through the use of screening and selection markers

Hani Boshra, Mahder Teffera, Jinxing Cao, and Shawn Babiuk

12. Using Rift Valley fever virus as a vector platform for the expression of ruminant disease antigens

Sandra Moreno Belen Borrego and Alejandro Brun

13. Generation and characterization of single-cycle infectious canine Influenza A Virus (sciCIV) and its use as vaccine platform

Aitor Nogales, Kevin Chiem, Michael Breen, Marta L DeDiego, Colin R Parrish, and Luis Martinez-Sobrido

14. Reverse genetics for influenza A and B viruses driven by swine polymerase I promoter.

Brittany Seibert, Stivalis Cardenas-Garcia, Daniela Rajao, and Daniel R. Perez

15. Analysis of the cellular immune responses to vaccines

Nicholas Svitek, Evans L. N. Taracha, Rosemary Saya, Elias Awino, Vish Nene, and Lucilla Steinaa