Main description:

This volume discusses different approaches to workflows for large volume electron microscopy - from preparation of samples to their imaging in a variety of microscopes - in some cases also applying correlative techniques. The chapters in this book cover topics such as correlative super resolution and electron microscopy to detect molecules in their native cellular context; low-threshold access to serial section arrays; improving serial blockface SEM by focal charge compensation; FIBSEM analysis of interfaces between hard technical devices and soft neuronal tissue; and image processing for volume electron microscopy. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Volume Microscopy: Multiscale Imaging with Photons, Electrons, and Ions is a valuable resource for novice and expert scientists interested in learning more about this evolving field.

Contents:

Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1. Correlative Super Resolution and Electron Microscopy to Detect Molecules in their Native Cellular Context
Tyler Ogunmowo, Sumana Raychaudhuri, Grant Kusick, Shuo Li, and Shigeki Watanabe

2. Multi-Color Super-Resolution Microscopy: Revealing the Nano-World of Astrocytes In Situ
Janosch P. Heller, James P. Reynolds, and Dmitri A. Rusakov

3. High Resolution Molecular Imaging and Its Applications in Brain and Synapses
Nhu T. N. Phan and Silvio O. Rizzoli

4. Advancing Array Tomography to Study the Fine Ultrastructure of Identified Neurons in Zebrafish (Danio rerio)
Marlene Strobel, Frederik Helmprobst, Martin Pauli, Manfred Heckmann, Christina Lillesaar, and Christian Stigloher

5. A Low-Tech Approach to Serial Section Arrays
Waldemar Spomer, Andreas Hofmann, Lisa Veith, and Ulrich Gengenbach

6. Large Volumes in Ultrastructural Neuropathology Imaged by Array Tomography of Routine Diagnostic Samples
Irene Wacker, Carsten Dittmayer, Marlene Thaler, and Rasmus R. Schroeder

7. Correlative Ultrastructural Analysis of Functionally Modulated Synapses using Automated Tape-Collecting Ultramicrotome and SEM Array Tomography
Ye Sun, Connon Thomas, Takayasu Mikuni, Debbie Guerrero-Given, Ryohei Yasuda, and Naomi Kamasawa

8. Large-Scale Automated Serial Section Imaging using a Multi-Beam Scanning Electron Microscope
Anna Lena Eberle and Tomasz Garbowski

9. Improving Serial Blockface SEM by Focal Charge Compensation
Ann-Katrin Unger, Ralph Neujahr, Chris Hawes, and Eric Hummel

10. Using X-Ray Microscopy to Increase Targeting Accuracy in Serial Block-Face Scanning Electron Microscopy
Eric A. Bushong, Sebastien Phan, and Mark H. Ellisman

11. FIBSEM Analysis of Interfaces between Hard Technical Devices and Soft Neuronal Tissue
Antje Biesemeier, Birgit Schroeppel, Wilfried Nisch, and Claus J. Burkhardt

12. Transforming FIB-SEM Systems for Large Volume Connectomics and Cell Biology
C. Shan Xu, Song Pang, Kenneth J. Hayworth, and Harald F. Hess

13. Image Processing for Volume Electron Microscopy
Joergen Kornfeld, Fabian Svara, and Adrian A. Wanner

14. Forget about Electron Micrographs: A Novel Guide for using 3D Models for Quantitative Analysis of Dense Reconstructions
Daniya J. Boges, Marco Agus, Pierre J. Magistretti, and Corrado Cali

Subject Index List...