Main description:

This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.

Contents:

Part I

Chapter 1 PRDM14, a zinc finger protein, regulates cancer stemness

Chapter 2 Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins

Chapter 3 Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation

Chapter 4 Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology

Chapter 5 Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers

Chapter 6 Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins

Chapter 7 Directed evolution of targeted recombinases for genome engineering

Chapter 8 Optical recording of cellular zinc dynamics with zinc finger based biosensors

Chapter 9 Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins

Chapter 10 Genome editing of MSCs as a platform for cell therapy

Part II

Chapter 11 Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells

Chapter 12 Selection and characterization of DNA aptamers against FokI nuclease domain

Chapter 13 An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs

Part III

Chapter 14 Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering

Chapter 15 Gene editing in channel catfish via double electroporation of zinc finger nucleases

Chapter 16 Delivery of mtZFNs into early mouse embryos

Chapter 17 Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain

Chapter 18 In-vivo applications of cell-penetrating zinc finger transcription factors

Chapter 19 Manufacturing and delivering genome-editing proteins