Main description:

This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols, Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.

Contents:

Part I: Production and Design

1. Antibiotics - precious goods in changing times

Peter Sass

2. A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycetes Culture Supernatants

Anika Rutten, Wolfgang Wohlleben, Lena Mitousis, and Ewa Maria Musiol-Kroll

3. Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals

Benjamin-Torres Salazar, Anna Lange, Laura Camus, and Simon Heilbronner

4. Production of Antimicrobial Compounds by Homologous and Heterologous Expression

I Dewa M. Kresna, Zerlina G. Wuisan, and Till F. Schaberle

5. Isolation and Purification of Natural Products from Microbial Cultures

Thomas Schafhauser and Andreas Kulik

6. Structure Elucidation of Antibiotics

Julia Moschny, Georgios Daletos, Peter Proksch, and Chambers C. Hughes

7. Computer-Aided Drug Design - An update

Wenbo Yu,David J. Weber, and Alexander D. MacKerell, Jr.

8. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents

Alexander Zipperer, Jasmin Scheurer, and Dorothee Kretschmer

Part II: Mode of Action and Resistance

9. Microscopy-based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action

Cruz L. Matos de Opitz and Peter Sass

10. Expansion Microscopy of Bacillus subtilis

Viola Middelhauve, Jan-Peter Siebrasse and Ulrich Kubitscheck

11. Tracking global and local changes in membrane fluidity through fluorescence spectroscopy and microscopy

Madeleine Humphrey, Ireny Abdelmesseh Nekhala, Kathi Scheinpflug, Oxana Krylova, Ann-Britt Schafer, Jessica A. Buttress, Michaela Wenzel, and Henrik Strahl

12. Quantitative analysis of microscopy data to evaluate bacterial responses to antibiotic treatment

Dominik Brajtenbach, Jan-Samuel Puls, Cruz L. Matos de Opitz, Peter Sass, Ulrich Kubitscheck, and Fabian Grein

13. Application of a Bacillus subtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds

Carolin Martina Kobras, Sali May Morris, Thorsten Mascher, and Susanne Gebhard

14. Determination of Bacterial Membrane Impairment by Antimicrobial Agents

Miriam Fuerst-Wilmes and Hans-Georg Sahl

15. A colorimetric assay to identify and characterize bacterial primase inhibitors

Allan H. Pang and Oleg V. Tsodikov

16. Cell-based fluorescent screen amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation Matteo Raneri, Emilio Alvarez-Ruiz, Danuta Mossakovska, and Federica Briani

17. Bacterial two component systems: Overexpression, purification in vitro and in vivo inhibitor screens

Alina Dietrich, Mike Gajdiss, Michael Turck, Ian Monk, and Gabriele Bierbaum

18. Sample preparation for mass-spectrometry based absolute quantification of bacterial proteins in antibiotic stress research Sandra Maass, Minia Antelo Varela, Florian Bonn, and Doerte Becher

19. Elemental analysis for the characterization of antimicrobial effects

Christoph H. R. Senges and Julia E. Bandow

20. label-free quantitation of ribosomal proteins from bacillus subtilis for antibiotic research

Sina Schakermann, Pascal Dietze, and Julia E. Bandow

21. Functional Metagenomics to Study Antibiotic Resistance

Bejan Mahmud, Manish Boolchandani, Sanket Patel, and Gautam Dantas