Main description:

This volume aims to help new investigators to the field by providing them with the protocols needed to set up clinically relevant animal models of fungal infection, as well as methods for in vivo immunology.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Antifungal Immunity: Methods and Protocols aims to be comprehensive guide for researchers in the field

Contents:

1. Quantifying the mechanical properties of yeast Candida albicans using atomic force microscopy-based force spectroscopy

Christopher R. Jones, Zhenyu Jason Zhang, and Hung-Ji Tsai

2. Standardization of Galleria mellonella as an infection model for Malassezia furfur and Malassezia pachydermatis

Maritza Torres and Adriana Marcela Celis Ramirez

3. Mouse organotypic brain slice cultures: a novel model for studying neuroimmune responses to cryptococcal brain infections

Amalia N. Awala, Maahir Kauchali, Anja de Lange, Emily Ruth Higgitt, Tshepiso Mbangiwa, Joseph V. Raimondo, and Rachael Dangarembizi

4. Zebrafish larvae as an experimental model of cryptococcal meningitis

Chalakova ZP and Johnston SA

5. Immunological Analysis of Cryptococcal Meningoencephalitis in a Murine Model

Jintao Xu, Kristie Goughenour, W. Rex Underwood, and Michal A Olszewski

6. Mouse Model of Latent Cryptococcal Infection and Reactivation

Ko Sato and Kazuyoshi Kawakami

7. Adoptive transfer of Cryptococcus neoformans-specific CD4 T-cells to study anti-fungal lymphocyte responses in vivo

Man Shun Fu, Kazuyoshi Kawakami, and Rebecca A. Drummond

8. Meningeal whole mounts for imaging CNS fungal infection

Sofia Hain Porter and Rebecca A. Drummond

9. An antibiotic-free model of Candida albicans colonisation of the murine gastrointestinal tract

Simon Vautier

10. An experimental model of chromoblastomycosis caused by Fonsecaea

sp species

Anamelia L Bocca and Isaque Medeiros Siqueira

11. Modelling Chronic Coccidioidomycosis in Mice

Lisa F. Shubitz, Christine D. Butkiewicz, and Hien T. Trinh

12. Mouse models of phaeohyphomycosis

Yi Zhang and Ruoyu Li

13. Genetic mouse models of Pneumocystis pneumonia

J. Claire Hoving, Ferris T. Munyonho, and Jay K. Kolls

14. Mouse models of Mucormycosis

Ilse D. Jacobsen

15. Bioluminescence imaging, a powerful tool to assess fungal burden in live mouse models of infection

Agustin Resendiz-Sharpe, Eliane Vanhoffelen, and Greetje Vande Velde

16. Micro-Computed Tomography to visualize and quantify fungal infection burden and inflammation in the mouse lung over time

Eliane Vanhoffelen, Agustin Resendiz-Sharpe, and Greetje Vande Velde