Main description:

Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of antibiotic issues and answer the worldwide demand for novel antibiotics therapies. Beginning with a review chapter that guides the reader through the worldwide demand for novel antibiotics therapies, the volume continues with sections covering new screening procedures and environmental sources, advances in analytical, microbiological, and biotechnological methodologies, antibiotic production and antibiotic resistances, as well as considerations of drug trials and clinical concerns regarding multi-resistant patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Antimicrobial Therapies: Methods and Protocols provides a reference source for health, laboratory, and industrial professionals, as well as for graduate students in a number of bio-sanitary disciplines, including medicine, nursery, biotechnology, veterinary, microbiology, genetics, molecular biology, nutrition, farming, and more.

Chapter "14" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Contents:

Part I: General Overview

1. Worldwide Clinical Demand for Antibiotics: Is It a Real Countdown?

Carlos Barreiro and Jose-Luis Barredo

Part II: Antibiotic Screening

2. Collection, Extraction, and In Vitro Antibacterial Evaluation of Plants Used in Traditional Medicine

Francois Chassagne and Cassandra Leah Quave

3. Microbial Isolation and Characterization of New Antibiotic-Producer Strains from Decayed Wood

Oscar Velasco-Rodriguez, Mariana Fil, Laura Garcia-Calvo, Katarina Kosalkova, and Carlos Barreiro

4. Characterization of Extremophilic Actinomycetes Strains as Sources of Antimicrobial Agents

Diyora Abdukhakimova, Zhannur Markhametova, Saikal Shamkeeva, Ainur Zhulamanova, Lyudmila Trenozhnikova, Vladimir Berezin, and Azliyati Azizan

5. Dilution-To-Extinction Platform for the Isolation of Marine Bacteria Producing Antitumor Compounds

Xulio Benitez, Jesus Garcia, Elena G. Gonzalez, and Fernando de la Calle

Part III: Methodologies

6. Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species

Jan Kormanec, Bronislava Rezuchova, and Renata Novakova

7. Antibacterial Assay in a Screening Project

Francisco Romero and Antonio Fernandez

8. Screening of Antibiotic Gene Clusters in Microorganisms Isolated from Wood

Mariana Fil, Oscar Velasco-Rodriguez, Laura Garcia-Calvo, Alberto Sola-Landa, and Carlos Barreiro

9. Methods in Screening Antiviral Drugs Against Enterovirus 71

Nadia Abubakar Omar Abubakar Bajaber and Babu Ramanathan

10. High-Efficiency Electroporation for Genetic Improvement of Fungal Strains

Jessica Cruz-Ramon, Francisco J. Fernandez, and Francisco Fierro

11. Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC

Katarina Kosalkova, Isabel-Clara Sanchez-Orejas, Laura Cueto, and Carlos Garcia-Estrada

12. Screening Fermentation and Extract Generation

Francisco Romero and Antonio Fernandez

13. A Simple Antifungal Assay for Testing Actinomycetes and Other Microbial Extracts

Maria A. Vinuesa and Antonio Fernandez

14. A User Guide for the Identification of New RiPP Biosynthetic Gene Clusters Using a RiPPER-Based Workflow

Alaster D. Moffat, Javier Santos-Aberturas, Govind Chandra, and Andrew W. Truman

15. Novel Methods to Identify Oxidative Stress-Producing Antibiotics

Alvaro Mourenza, Jose A. Gil, Luis M. Mateos, and Michal Letek

16. Coupled Transcriptomics for Differential Expression Analysis and Determination of Transcription Start Sites: Design and Bioinformatics

Antonio Rodriguez-Garcia, Alberto Sola-Landa, and Rosario Perez-Redondo

17. High Throughput Screening Assays for Dengue Antiviral Drug Development

Shobana Gabriel Jabanathan, Low Zhao Xuan, and Babu Ramanathan

18. Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline

Lucija Slemc, Spela Pikl, Hrvoje Petkovic, and Martina Avbelj

Part IV: Antibiotic Production

19. Production, Detection, Extraction, and Quantification of Polyene Antibiotics

Eva G. Barreales and Jesus F. Aparicio

20. Strain Improvement Program of Streptomyces roseosporus for Daptomycin Production

Sara Rubio, Sonia Martinez-Camara, Juan Luis de la Fuente, Marta Rodriguez-Saiz, and Jose-Luis Barredo

Part V: Antibiotic Resistance

21. Comparative Metabolomics Revealing the Metabolic Responses of Pathogenic Bacteria to Different Antibiotics

Fouad Choueiry, Xiaowei Sun, and Jiangjiang Zhu

22. Proteomic Analysis of Antibiotic Susceptibility in Enterobacteriaceae

Sarah L. Keasey, Bryan M. Zhao, Robert G. Ulrich, and Moo-Jin Suh

23. Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose-Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi)

Samantha Donnellan, Carmen Martinez-Rodriguez, Ghaith Aljayyoussi, and Giancarlo A. Biagini

Part VI: Market

24. Clinical Drug Trials: The Path to the Patient

Cristina Diez Pascual

25. Antibiotics Therapy in the Multi-Resistant Patient

Marta Maria Blanco-Nistal and Jesus Antonio Fernandez-Fernandez