Main description:

This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients.

Contents:

1. Autoantibodies as Diagnostic Tools

Gunnar Houen

2. Non-specific Binding in Immunoassays for Autoantibodies

Gunnar Houen

3. Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on HEp-2 Cells

Alessandra Dellavance and Luis Eduardo Coelho Andrade

4. Detection of Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence

J. J. B. C. van Beers, J. Vanderlocht, C. Roozendaal, and Jan G. M. C. Damoiseaux

5. Determination of Subset-Restricted Anti-Neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry

Firoozeh Amirbeagi, Amanda Welin, Pontus Thulin, and Johan Bylund

6. Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry

Jehan Alam, Sumin Jeon, and Youngnim Choi

7. Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry

Satoshita Yamashita and Nozomu Tawara

8. Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay

Hanna Davideit, Annekathrin Haberland, Sabine Bartel, Sarah Schulze-Rothe, Johannes Muller,and Katrin Wenzel

9. Determination of Autoantibodies to Salivary Gland Antigens

Li Cui, Xinyuan Zhao, and Shen Hu

10 Measurement of autoantibodies to the gastric H+,K+-ATPase (ATP4A/B) using a Luciferase Immuno Precipitation System (LIPS)

Edith Lahner, Ilaria Marzinotto, Cristina Brigatti, Howard Davidson, Janet Wenzlau, Lorenzo Piemonti, Bruno Annibale, and Vito Lampasona

11. Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay

Jacqueline Keyhani and Ezzatollah Keyhani

12. Determination of Autoantibodies to Neutrophil Granule Constituents by Solid Phase Immunoassay

J. Vanderlocht, J.J.B.C. van Beers, P.C. Limburg, Jan G. M. C. Damoiseaux, and C. Roozendaal

13. Determination of Autoantibodies to C3 Nephritic Factors by ELISA

Lillemor Skattum

14. Determination of Anti-C1q Autoantibodies by Western Blot

Anci Verlemyr, Lennart truedsson, and Lillemor Skattum

15. Anti-Factor H Autoantibodies Assay by ELISA

Marie Senant and Marie-Agnes Durey

16. Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay

Zhiyuan Zhao, Yong Gu, Dongmei Miao, Eric Hoffmeyer, Yu Liu, and Liping Yu

17. Determination of CRP Autoantibodies by SPR Immunoassay

Li Qiu-yu and Li Hai-yun

18. Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis

Felicia Real-Fernandez, Federico Pratesi, Paola Migliorini, and Paolo Rovero

19. Detection of SSA and SSB Autoantibodies by Enzyme-Linked Immunosorbent Assay

Nicole Hartwig Trier

20. Assessment of Peptidylarginine Deiminase Activity by ELISA using Human Fibrinogen as Substrate

Dres Damgaard and Claus H. Nielsen

21. The Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay

Nicole H. Trier

22. Determination of Autoantibodies by Line Blotting

Louise Sternbaek, Tina Friis, and Gunnar Houen

23. Determination of Rheumatoid Factors by ELISA

Nicole H. Trier and Gunnar Houen

24. Detection of autoantibodies to complement components by surface plasmon resonance-based technology

Remi Noe, Sophie Chauvet, Shambhuprasad K. Togarsimalemath, Maria Chiara Marinozzi, Maria Radanova, Vasil V. Vasilev, Veronique Fremeaux-Bacchi, Marie-Agnes Dragon-Durey, and Lubka T. Roumenina