Main description:

This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets.

Contents:

1. Structure determination of bacterial polysaccharides 2. Biosynthesis of complete O antigen repeating units in pathogenic bacteria

3. S-layer biosynthesis

4. Synthesis of mammalian glycoconjugates in Neisseria

<5. Polymerizing Mannosyltransferases

6. Chain length and termination control

7. O antigen Polymerases

8. Polysialyltransferases in bacteria

9. Polymerizing Sialyl-hexose transferase

10. Kdo-transferases and capsule biosynthesis

10. Production of legionaminic acids by sialyltransferases

11. Outer core L-rhamnosyltransferases that control O antigen attachment

12. Production of human blood group epitopes in E.coli

13. Chemoenzymatic synthesis of undecaprenyl phosphate analogs

14. Inhibitor of undecaprenyl diphosphate synthase

15. Chemical synthesis of TDP-Rha

16. Synthesis of TDP-L-Rha analogs

17. Simplified synthesis of nucleotide sugars

18. Inhibition of bacterial glycosyltransferases

19. Glycosyltransferase protein structure determination

20. Crystal Structure of MurNAc- 1-P Uridylyltransferase MurU

21. O-acetylation in peptidoglycans

22. Assays of nucleotide sugar-dependent glycosyltransferases

23. Mechanism of Staphylococcus aureus cell wall teichoic Acid -glycosyltransferase involved in Methicillin resistance.

24. Mycobacterial arabinosyl-transferase activity

25. Immunogenicity of bacterial polysaccharides

26. Receptor binding of bacteriophages

27. Bacterial N-glycosylation

28. Protein O-glycosylation in bacteria

29. Role of polysaccharide in biofilm formation

30. O antigens and biofilms