Main description:

The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc.

The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing.

Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique.

The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

Contents:

Part I: Basic Laboratory Techniques in Histopathology Laboratory
Chapter 1: Fixation of histology samples: Principles, methods and types of fixatives Chapter 2: Processing of tissue in histopathology laboratoryChapter 3: Embedding of tissue in histopathologyChapter 4: Decalcification of bony and hard tissue for histopathology processingChapter 5: Tissue Microtomy: principle and procedureChapter 6: Frozen section: Principle and procedureChapter 7: Staining principle and general procedure of staining of the tissueChapter 8: Haematoxylin and Eosin stain of the tissue sectionChapter 9: Special Stains for the carbohydrate, protein, lipid, nucleic acid and pigmentsChapter 10: Connective tissue stain: Principle and procedureChapter 11: Amyloid stainingChapter 12: Stains for the Microbial Organisms
Part II: Basic Laboratory Techniques in Cytology Laboratory
Chapter 13: Cytology sample procurement, fixation and processingChapter 14: Routine staining in cytology laboratoryChapter 15: Basic technique of fine needle aspiration cytology
Part III: Advanced Techniques in Histology and Cytology Laboratories
Chapter 16: Immunocytochemistry in histology and cytologyChapter 17: Flow cytometry: Basic principles, procedure and applications in pathologyChapter 18: Digital pathology and whole slide imaging Chapter 19: Automation and liquid based cytology in cytology sampleChapter 20: Polymerase chain reaction: Principle, technique and applications in pathology Chapter 21: Fluorescent in situ hybridisation techniques in pathology: Principle, technique and applicationsChapter 22: Tissue microarray in pathology: Principal, technique and applications Chapter 23: Sanger sequencing and next generation gene sequencing: Basic principles and applications in pathologyChapter 24: Liquid biopsy: Basic principles, techniques and applications Chapter 25: Artificial neural network in Pathology: Basic principles and applications
Part IV: Microscopy, quality control and laboratory organization
Chapter 26: Light microscope and other different microscopesChapter 27: Fluorescence and confocal microscope: Basic principles and applications in pathologyChapter 28: Electron microscopy: Principle, components, optics and specimen processingChapter 29: Quality control and laboratory organization Chapter 30: Laboratory safety and laboratory waste disposalChapter 31: Multiple choice questions