Main description:

This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.

Contents:

Dedication...Preface...Table of Contents...Contributing Authors...
1. Engineering Citric-Acid Based Porous Scaffolds for Bone RegenerationJacqueline J. Masehi-Lano and Eun Ji Chung
2. Multifunctional Self-Assembling Peptide-Based Micelles for Targeted Intracellular Delivery: Design, Physicochemical Characterization, and Biological AssessmentYejiao Shi, Ran Lin, Honggang Cui, and Helena S. Azevedo
3. Electrospinning Functionalized Polymers for Use as Tissue Engineering ScaffoldsLesley W. Chow
4. Low-Temperature Deposition Modelling for CP Scaffolds with Controlled Bimodal PorosityE. Papastavrou, P. Breedon, and D. Fairhurst
5. Three-Dimensional Hydrogel-Based Culture to Study the Effects of Toxicants on Ovarian FolliclesHong Zhou and Ariella Shikanov
6. Layer-by-Layer Engineered Polymer Capsules for Therapeutic DeliveryRona Chandrawati
7. Controlling Fibrin Network Morphology, Polymerization, and Degradation Dynamics in Fibrin Gels for Promoting Tissue RepairErin P. Sproul, Riley T. Hannan, and Ashley C. Brown
8. Biofunctionalization of Poly(acrylamide) GelsJulieta I. Paez, Aleeza Farrukh, Oya Ustahuseyin, and Aranzazu del Campo
9. Synthetic PEG Hydrogel for Engineering the Environment of Ovarian FolliclesUziel Mendez, Hong Zhou, and Ariella Shikanov
10. Engineering Human Neural Tissue by 3D BioprintingQi Gu, Gordon G. Wallace, and Jeremy M. Crook
11. High-Throughput Formation of Mesenchymal Stem Cell Spheroids and Entrapment in Aliginate HydrogelsCharlotte E. Vorwald, Steve S. Ho, Jacklyn Whitehead, and J. Kent Leach
12. Crimped Electrospun Fibers for Tissue EngineeringPen-hsiu Grace Chao
13. In Vitro Model of Macrophage-Biomaterial InteractionsClaire E. Witherel, Pamela L. Graney, and Kara L. Spiller
14. Synthesis of Self-Assembling Peptide-Based Hydrogels for Regenerative Medicine Using Solid-Phase Peptide SynthesisE. Thomas Pashuck
15. H2S Delivery from Aromatic Peptide Amphiphile HydrogelsKuljeet Kaur, Yun Qian, and John B. Matson