Main description:

This volume provides detailed technical protocols on current biomedical technologies and examples of their applications and capabilities. Chapters focus on molecular and cellular analytical methods, experimental new drug delivery approaches, guided surgery, implants and tissue engineering. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and practical, Biomedical Engineering Technologies, Volume 2 provides technical details in descriptions of major technologies by experts in the field.

Contents:

Part I: Molecular and Cellular analysis and manipulation

1. Development of a Multi-Target Protein Biomarker Assay for Circulating Tumor Cells

Diya Li, Ceming Wang, Yingjia Ni, Yaoping Liu, Wei Wang, Siyuan Zhang, Hsueh-Chia Chang, and Satyajyoti Senapati

2. Method to Isolate Dormant Cancer Cells from Heterogeneous Populations

Julian A. Preciado, and Alptekin Aksan

3. Label-Free Morphological Phenotyping of in vitro 3D Micro Tumors

Zoe Moscato, Devina Jaiswal, Krishna Dixit, Cooper J. Langanis, Kevin P. Claffey, and Kazunori Hoshino

4. High-throughput microenvironment microarray (MEMA) (file #high-resolution imaging

Tiina A Jokela, Michael E Todhunter, and Mark A LaBarge

5. Real-Time Analysis of AKT Signaling Activities at Single-Cell Resolution Using Cyclic Peptide-Based Probes

Fei Ji, Siwen Wang, Shiqun Shao, Priyanka Sarkar, and Min Xue

6. Microfluidic device technologies for digestion, disaggregation, and filtration of tissue samples for single cell applications

Jeremy A. Lombardo, and Jered B. Haun

7. Microdissection methods utilizing single cell subtype analysis and the impact on precision medicine

Donald J. Johann, Jr., Sarah Laun, Owen Stephens, Robert Weigman, , Ikjae Shin, , Adam Roberge, Meeiyueh Liu, Valerie Greisman, Mathew Steliga, Jason Muesse, , Erich Peterson, , Michael R. Emmert-Buck and Michael A. Tangrea,

8. Functionalized lineage tracing for the study and manipulation of heterogeneous cell populations

Andrea Gardner, Daylin Morgan, Aziz Al'Khafaji, and Amy Brock

9. Fluorescence lifetime imaging probes for cell-based measurements of enzyme activity

Sampreeti Jena, and Laurie L. Parker

10. Assessment of intracellular GTP levels using genetically encoded fluorescent sensors'

Anna Bianchi-Smiraglia, and Mikhail. Nikiforov

11. Node-Pore Sensing for characterizing cells and extracellular vesicles

Thomas Carey, Brian Li, and Lydia L. Sohn

12. Affinity-Based Enrichment of Extracellular Vesicles with Lipid Nanoprobes

Yuan Wan, Mackenzie Maurer, and Si-Yang Zheng

13. Droplet magnetofluidic assay platform for quantitative methylation-specific PCR Alejandro Stark, Alexander Trick, Thomas R Pisanic II, and Tza-Huei Wang

14. Droplette: A Platform Technology to Directly Deliver Nucleic Acid Therapeutics and Other Molecules into Cells and Deep into Tissue Without Transfection Reagents

Bao Lin Quek, Rathi L Srinivas, and Madhavi P Gavini

15. Molecular imaging of HER2 in patient tissues with touch prep-quantitative single molecule localization microscopy

Devin L. Wakefield, Steven J. Tobin, Daniel Schmolze, and Tijana Jovanovic-Talisman

16. Microchip free-flow electrophoresis for bioanalysis, sensing and purification

William E. Arter, Kadi L. Saar, Therese W. Herling, and Tuomas P. J. Knowles

17. Green Chemistry Preservation and Extraction of Biospecimens for Multi-omic Analyses

Andrey P. Tikunov, Jeremiah D. Tipton, Timothy J. Garrett, Sachi V. Shinde, Hong Jin Kim, David A. Gerber, Laura E. Herring, Lee M. Graves, and Jeffrey M. Macdonald

18. TdT-UTP DSB End Labeling (TUDEL), for Specific, Direct in situ Labeling of DNA Double Strand Breaks

Julian Lutze, Sara E Warrington, and Stephen J. Kron

19. Ligand-directed GPCR Antibody Discovery

Qi Zhao, Amanda Chapman, Yan Huang, Mary Ferguson, Shannon McBride, Meghan Kelly, Michael Weiner, and Xiaofeng Li

20. Self-Induced Back-Action Actuated Nanopore Electrophoresis (Sane) (File #Sensor For Label-Free Detection Of Cancer Immunotherapy-Relevant Antibody-Ligand Interactions

Sai Santosh Sasank Peri, Muhammad Usman Raza, Manoj K. Sabnani, Soroush Ghaffari, Susanne Gimlin, Debra D. Wawro, Jung Soo Lee, Min Jun Kim, Jon Weidanz, and George Alexandrakis

21. Incorporating, quantifying, and leveraging noncanonical amino acids in yeast

Jessica T. Stieglitz, and James A. Van Deventer

22. Nuclease-assisted, multiplexed minor-allele enrichment: application in liquid biopsy of cancer

Fangyan Yu, Ka Wai Leong, and G. Mike Makrigiorgos

23. Implementation of Ion Mobility Spectrometry-Based Separations in Structures for Lossless Ion Manipulations (SLIM)

Adam L. Hollerbach, Christopher R. Conant, Gabe Nagy, and Yehia M. Ibrahim

24. Pleural Effusion Aspirate for use in 3D Lung Cancer Modeling and Chemotherapy Screening

Andrea Mazzocchi, Anthony Dominijanni, and Shay Soker

25. Using Optical Tweezers to Dissect Allosteric Communication Networks in Protein Kinases

Yuxin Hao and Rodrigo Maillard

Part II: Therapeutics Technologies

26. Focused ultrasound-mediated intranasal brain drug delivery technique (FUSIN)

Dezhuang Ye, and Hong Chen

27. Extracellular pH mapping as therapeutic readout of drug delivery in glioblastoma

John J. Walsh, and Fahmeed Hyder

28. Charge-Based Multi-Arm Avidin Nano-construct as a Platform Technology for Applications in Drug Delivery

Tengfei He, Chenzhen Zhang, and Ambika G. Bajpayee

29. Chemical Modification of Proteins and Their Intracellular Delivery Using Lipidoid Nanoparticles

Yamin Li, Zachary Glass, and Qiaobing Xu

30. Generation of Membrane-derived Nanovesicles by Nitrogen Cavitation for Drug Targeting Delivery and Immunization

Jin Gao, Mindy Lee, Xinyue Dong, and Zhenjia Wang

31. Laboratory-scale production of sterile targeted microbubbles

Guixin Shi, and Yu-Tsueng Liu

32 (file #47). Adeno-associated viral vector immobilization and local delivery from bare metal surfaces. Ben B. Pressly, Bahman Hooshdaran, Ivan S. Alferiev, Michael Chorny, Robert J. Levy, and Ilia Fishbein

33. Decellularization and Recellularization Methods for Avian Lungs: An Alternative Approach for Use in Pulmonary Therapeutics

Alicia E. Tanneberger, Daniel J. Weiss, and Juan J. Uriarte

34. Methods for Forming Human Lymphatic Microvessels In Vitro and Assessing Their Drainage Function

Joe Tien, and Usman Ghani

35. Natural Polymer Based Micro-Nanostructured Scaffolds for Bone Tissue Engineering

Sara Katebifar, Devina Jaiswal, Michael R. Arul, Sanja Novak, Jonathan Nip, Ivo Kalajzic, Swetha Rudraiah, and Sangamesh G. Kumbar

36. Biodegradable electrospun nanofibrous scaffolds for bone tissue engineering

Aneela Anwar, Daniel Jerome Petrino Jr., Nicole Van Alstine, and Xiaojun Yu

37. Bio-Tribometer for the Assessment of Cell and Tissue Toxicity of Orthopedic Metal Implant

Debris Simona Radice, and Markus A. Wimmer

38. Methods for Quantifying Neutrophil Extracellular Traps on Biomaterials

Allison E. Fetz, William E. King III, Benjamin A. Minden-Birkenmaier, and Gary L. Bowlin

39. In Vivo Imaging of Implanted Hyaluronic Acid Hydrogel Biodegradation

Shreyas Kuddannaya, Wei Zhu, and Jeff W.M. Bulte

40. Computational modeling and simulation to quantify the effects of obstructions on the performance of ventricular catheters used in hydrocephalus treatment

Stephanie C. TerMaath, Douglas L. Stefanski, and James A. Killeffer

41. Selection of Cancer Stem Cell--targeting Agents Using Bacteriophage Display

Austin R. Prater, and Susan L. Deutscher

42. Nano-Scintillator-Based X-Ray Induced Photodynamic Therapy

Benjamin Cline, and Jin Xie

43. Methods to measure the inhibition of ABCG2 transporter and ferrochelatase activity to enhance aminolevulinic acid-protoporphyrin IX fluorescence-guided tumor detection and resection

Matthew Mansi, Richard Howley, and Bin Chen

44. Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug-Target Engagement

Marien Ochoa, Alena Rudkouskaya, Jason T. Smith, Xavier Intes, and Margarida Barroso

45. Tumor in vivo Imaging with a New Peptide-based Fluorescent Probe

Samer Naffouje, Masahide Goto, Ingeun Ryoo, Albert Green, Tapas K. Das Gupta, and Tohru Yamada

46. Thermal Ablation Treatment for Cervical Precancer (Cervical Intraepithelial Neoplasia grade 2 or higher [CIN2+])

Montserrat Soler, Rachel Masch, Rakiya Saidu, and Miriam Cremer

47. Employing Novel Porcine Models of Subcutaneous Pancreatic Cancer to Evaluate Oncological Therapies

Alissa Hendricks-Wenger, Margaret A Nagai-Singer, Kyungjun Uh, Eli Vlaisavljevich, Kiho Lee, and Irving C Allen