Main description:

This volume focuses on experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). This book is organized into six parts: Part One is an overview of the physiology of BBB; Part Two explores in vitro cell models to study the BBB; Part Three discusses techniques in vivo and ex vivo models to evaluate BBB in Drosophila melanogaster, Zebrafish, and rodents; Part Four looks at permeability, influx, efflux transportation, and drug delivery through the BBB; Part Five talks about various invasive and non-invasive imaging techniques to study BBB; and Part Six describes how molecular biomarkers are used to look at the integrity or dysfunction of the BBB. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Blood-Brain Barrier is a valuable resource to aid both novice and experienced investigators with performing experiments using new and classic translational approaches.

Contents:

Series Preface...Preface...Table of Contents...Contributing Authors...
1. An Overview of the Blood-Brain BarrierTatiana Barichello, Allan Collodel, Rodrigo Hasbun, and Rodrigo Morales
2. Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and TherapeuticsBrain M. Kopec, Kavisha R. Ulapane, Mario E.G. Moral, and Teruna J. Siahaan
3. Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modelling of the Blood-Brain BarrierMalgorzata Burek and Carola Y. Foerster
4. In Vitro BBB Models: Working with Static Platforms and Microfluidic SystemsMohammad A. Kaisar, Vinay V. Abhyankar, and Luca Cucullo
5. In Vitro Cell Models of the Human Blood-Brain Barrier: Demonstrating the Beneficial Influence of Shear Stress in Brain Microvascular Endothelial Cell PhenotypeKeith D. Rochfort and Philip M. Cummins
6. Transepithelial/Transendothelial Electrical Resistance (TEER) to Measure the Integrity of Blood-Brain BarrierBalaji Srinivasan and Aditya Reddy Kolli
7. Cell Penetrating Peptides as Theranostics against Impaired Blood-Brain barrier Permeability: Implications for Pathogenesis and Therapeutics Treatment of Neurodegenerative DiseaseSwapna Bera and Anirban Bhunia
8. Microbial Translocation of the Blood-Brain BarrierCharles T. Spencer and Mireya G. Ramos-Muniz
9. Transport across the Choroid Plexus: How to Culture Choroid Plexus Cells and Establish a Functional Assay SystemSen Takeda and Keishi Narita
10. Drosophila as a Model to Study the Blood-Brain BarrierCameron Love and Brigitte Dauwalder
11. Zebrafish (Danio rerio) as a Viable Model to Study the Blood-Brain BarrierTianzhi Yang and Shuhua Bai
12. Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult RodentsFabricia Petronilho, Julia L. Goldman, and Tatiana Barichello
13. Experimental Tools to Study the Regulation and Function of the Choroid PlexusIsabel Goncalves, Telma Quintela, Ana Catarina Duarte, Peter Hubbard, Graca Baltazar, Christian Schwerk, Andrea Carmine Belin, Joana Tomas, and Cecilia Reis A. Santos
14. Techniques for Evaluating Efflux Transport of Radiolabeled Drugs and Compounds from the Cerebrospinal Fluid acorss the Blood-Cerebrospinal Fluid BarrierShin-ichi Akanuma, Yoshiyuki Kubo, and Ken-ichi Hosoya
15. In Vivo Analysis to Study Transport across the Blood-Retinal BarrierYoshiyuki Kubo, Shin-ichi Akanuma, and Ken-ichi Hosoya
16. Increasing BBB Permeability via Focused Ultrasound: Current Methods in Pre-Clinical ResearchDallan McMahon, Charissa Poon, and Kullervo Hynynen
17. Evaluation of Blood-Brain Barrier Permeability and Integrity in Juvenile Rodents: Dynamic Contrast-Enhanced (DCE), Magnetic Resonance Imaging (MRI), and Evan's Blue ExtravasationTrish Domi, Faraz Honarvar, and Andrea Kassner
18. Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital MicroscopyAline Silva de Miranda, Thiago Macedo Cordeiro, Milene Alvarenga Rachid, and Antonio Lucio Teixeira
19. Molecular Imaging of Blood-Brain Barrier Permeability in Preclinical Models Using PET and SPECTVijayasree V. Giridharan, Tatiana Barichello, and Sudhakar Selvaraj
20. Biomarkers for Microvascular Proteins Detection: Blood-Brain Barrier Injury and Damage MeasurementPavani Sayana, Jean Pierre Oses, Tatiana Barichello, and Vijayasree V. Giridharan