Main description:

This detailed volume explores numerous methods used in basic science laboratories to characterize cancer-related biomarkers, vital for better managing cancer burden, including cancer risk assessment, cancer diagnosis, determining cancer progression, and therapeutic response. From a radiography method to an examination of single-cell RNA-seq and computational analysis tools in cancer research, this book delves into many techniques that could provide valuable molecular information about the tumor and its microenvironment components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cancer Biomarkers: Methods and Protocols offers researchers multiple helpful ways to study cancer-associated molecular biomarkers.

Contents:

1. A Method of Bone-Metastatic Tumor Progression Assessment in Mice Using Longitudinal Radiography

Matthew R. Eber, Juan Miguel Jimenez-Andrade, Christopher M. Peters, and Yusuke Shiozawa

2. Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice

Susy Kim and Gagan Deep

3. Method to Development of PET Radiopharmaceutical for Cancer Imaging

Naresh Damuka and Kiran Kumar Solingapuram Sai

4. PET Use in Cancer Diagnosis, Treatment, and Prognosis

Naresh Damuka, Meghana Dodda, Kiran Kumar Solingapuram Sai

5. Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor

Leslimar Rios-Colon, Susy Kim, Yixin Su, Deepak Kumar, and Gagan Deep

6. Zebrafish Xenograft Model to Study Human Cancer

Ranganatha R. Somasagara and TinChung Leung

7. Assessing Oligomerization Status of Mitochondrial OXPHOS Complexes via Blue Native Page

Jordan Woytash, Joseph A Inigo, and Dhyan Chandra

8. In Vitro Cell Impedance Assay to Examine Antigen-Specific T-Cell-Mediated Melanoma Cell Killing to Support Cancer Immunotherapy Drug Discovery

Elizabeth R. Stirling and David R. Soto-Pantoja

9. Methods to Detect Nitric Oxide and Reactive Nitrogen Species in Biological Sample

Sharanjot Kaur, Kunj Bihari Gupta, Sandeep Kumar, Shishir Upadhyay, Anil Kumar Mantha, and Monisha Dhiman

10. Study of Rotary Cell Culture System Induced Microgravity Effects on Cancer Biomarkers

Ragini Singh and Rana P. Singh

11. A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons

Joshua J. Wheeler, John M. Davis, and Santosh K. Mishra

12. Zymography and Reverse Zymography for Testing Proteases and Their Inhibitors

Preety Choudhary, Vineet Kumar Mishra, and Snehasikta Swarnakar

13. Method of Preparation of Cigarette Smoke Extract to Assess Lung Cancer-Associated Changes in Airway Epithelial Cells

Hina Agraval, Jiten R. Sharma, and Umesh C.S. Yadav

14. Air-Liquid Interface Culture Model to Study Lung Cancer-Associated Cellular and Molecular Changes

Hina Agraval, Jiten R. Sharma, Neeraj Dholia, and Umesh C.S. Yadav

15. Co-Immunoprecipitation-Blotting: Analysis of Protein-Protein Interactions

Li Tan and Raghunatha R. Yammani

16. Methods to Assess Oxidative DNA Base Damage Repair of Apurinic/Apyrimidinic (AP) Sites Using Radioactive and Non-Radioactive Oligonucleotide-Based Assays

Kunj Bihari Gupta, Sharanjot Kaur, Monisha Dhiman, and Anil Kumar Mantha

17. Determining the Size Distribution and Integrity of Extracellular Vesicles by Dynamic Light Scattering

Mohammad Aslam Khan, Shashi Anand, Sachin Kumar Deshmukh, Seema Singh, and Ajay Pratap Singh

18. Characterization of Exosomal Surface Proteins by Immunogold Labeling

Yixin Su, Ashish Kumar, and Gagan Deep

19. Scanning Electron Microscopy of Giant Cells from Giant Cell Tumor of Bone

Asit Ranjan Mridha and Subhash Chandra Yadav

20. Raman Microscopy Techniques to Study Lipid Droplet Composition in Cancer Cells

Mariana C. Potcoava, Gregory L. Futia, Emily A. Gibson, and Isabel Schlaepfer

21. Surface Plasmon Resonance, a Novel Technique for Sensing Cancer Biomarker: Folate Receptor and Nanoparticles Interface

Santosh Kumar Singh and Rajesh Singh

22. Characterization of Tobacco Microbiome by Metagenomics Approach

R. Suresh Kumar, Nivedita Mishra, and Amit Kumar

23. Single-Cell RNA-Seq Technologies and Computational Analysis Tools: Application in Cancer Research

Qianqian Song and Liang Liu