Main description:

This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro.

Contents:

1. A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy

Laura Kutz, Tong Zhou, Qi Chen, and Hua Zhu

2. Remote Ischemic Pre-Conditioning (RIPC)

Kyung Eun Lee, Jongsoo Kim, Ki Ho Park, Jianjie Ma, and Hua Zhu

3. Mouse Corneal Transplantation

Peng Chen, Ki Ho Park, Liqiang Zhang, Alexandra R. Lucas, Heather L. Chandler, and Hua Zhu

4. Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant

Yanlin Ma and Hao Chen

5. Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade

Isabela R. Zanetti, Liqiang Zhang, Michelle Burgin, Jacquelyn Kilbourne, Jordan R. Yaron, David Fonseca, and Alexandra R. Lucas

6. Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion

Sanne L. Maas, Remco T.A. Megens, and Emiel P.C. van der Vorst

7. In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor

Sanne L. Maas and Emiel P.C. van der Vorst

8. The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins

Wesley M. Tierney and Ian B. Hogue

9. Flow Cytometry Analysis of Immune Cell Responses

Kavita R. Manhas and Joseph N. Blattman

10. Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence

Bruno Hernaez and Antonio Alcami

11. Protein Microarrays and Their Fabrication

Mahasish Shome and Joshua LaBaer

12. NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions

Vitor H. Pomin and Krishna Rajarathnam

13. Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions

Amrita Basu and Ryan J. Weiss

14. Isolation and Compositional Analysis of Glycosaminoglycans

Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss, and Parastoo Azadi

15. How to Design Peptides

Joseph Dodd-o, Amanda M. Acevedo-Jake, Abdul-Rahman Azizogli, Vikram Khipple Mulligan, and Vivek A. Kumar

16. Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins

Elham Torbati, Gabriella Stuart, Kurt Krause, Chris Brown, and Lyn Wise

17. Producing Biologics with Defined N-Glycosylation in Plants

Adrian Esqueda and Qiang Chen

18. Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery

Ryan Boyd, Liqiang Zhang, and Petra Fromme