Main description:

This book aims to provide scientists with tools and well-researched protocols to enable their research and to facilitate further progress in this common leukemia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Chronic Lymphocytic Leukemia: Methods and Protocols aims to accelerate research on chronic lymphocytic leukemia and further improvements in patient outcomes.

Contents:

1: Ex-Vivo signal Transduction Studies in Chronic Lymphocytic Leukemia

Karly-Rai Rogers-Broadway, Laura Karydis, Rachel C Dobson, and Andrew J Steele

2: Ex vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells

Burcu Aslan, Mary L. Ayres, and Varsha Gandhi

3: Culture and Harvest of CpG-stimulated Blood and Bone Marrow in Chronic Lymphocytic Leukemia

Cecelia R. Miller and Nyla A. Heerema

4: Analysis of Common Abnormalities seen in Chronic Lymphocytic Leukemia using Fluorescence in situ Hybridization

Reid G. Meyer and Daniel L. Van Dyke

5: Immunoglobulin Gene Analysis in Chronic Lymphocytic Leukemia

Andreas Agathangelidis, Richard Rosenquist, Frederic Davi, Paolo Ghia, Chrysoula Belessi, Anastasia Hadzidimitriou, and Kostas Stamatopoulos

6: Detection and Functional Analysis of TP53 Mutations in CLL

Sarka Pavlova, Jana Smardova, Nikola Tom, and Martin Trbusek

7: The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-mutant CLL

Tushar Murthy, Kiran V. Paul, Alexander C. Minella, and Manoj M. Pillai

8: Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and its Clinical Applications

Antonella Zucchetto, Erika Tissino, Alexander Chigaev, Tanja Nicole Hartmann, and Valter Gattei

9: In vitro Assay to Study CLL and Monocyte Interactions

Maria Teresa Sabrina Bertilaccio, Ronghua Zhang, Priyanka Banerjee, and Varsha Gandhi

10: Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells

Hima V. Vangapandu and Varsha Gandhi

11: Measurement of Leukemic B-cell Growth Kinetics in Patients with Chronic Lymphocytic Leukemia

Andrea N. Mazzarello, Mark Fitch, Marc K. Hellerstein, and Nicholas Chiorazzi

12: Assays on DNA Damage and Repair in CLL

Tzung-Huei Lai and Deepa Sampath

13: Method for Generating a Patient-Derived Xenograft Model of CLL

Shih-Shih Chen

14: Immunological Methods to Study Monoclonal Antibody Activity in Chronic Lymphocytic Leukemia

MJ Carter, LN Dahal, KLS Cleary, MJE Marshall, RR French, SA Beers, and MS Cragg

15: CRISPR/Cas9-based Gene Dropout Screens

Kai Wu and Sami N. Malek

16: Gene Disruption using CRISPR-Cas9 Technology

Nan Hu and Sami N. Malek

17: Minimal Residual Disease Quantification in Chronic Lymphocytic Leukemia: Clinical Significance and Flow Cytometric Methods

Sebastian Boettcher

18: Rare Variant Quantitation Using Droplet Digital PCR

Brian Parkin

19: Methods for Measuring ctDNA in Lymphomas

Davide Rossi, Adalgisa Condoluci, Valeria Spina, and Gianluca Gaidano

20: Measurement of miRNAs in Chronic Lymphocytic Leukemia Patient Samples by Quantitative Reverse Transcription PCR

Katrien Van Roosbroeck, Recep Bayraktar, and George A. Calin

21: Bioinformatics Analysis of Whole Exome Sequencing Data

Peter J. Ulintz, Weisheng Wu, and Chris M. Gates

22: Genome Sequencing and Analysis Methods in Chronic Lymphocytic Leukemia

Victor Quesada, Miguel Araujo-Voces, Jose G. Perez-Silva, Gloria Velasco, and Carlos Lopez-Otin

23: Characterization of Somatically-acquired Copy Number Alterations in Chronic Lymphocytic Leukemia using Shallow Whole Genome Sequencing

Helen Parker, Louise Carr, Sharma Syeda, Dean Bryant, and Jonathan C Strefford

24: High-throughput Sequencing of the T Cell Receptor Beta Chain Gene Repertoire in Chronic Lymphocytic Leukemia

Vlachonikola E, Vardi A, Stamatopoulos K, and Hadzidimitriou A