Main description:

For decades, the Clinical Virology Manual has been the definitive source of the latest information and procedures for the physician and the clinical laboratory virologist. This edition is thoroughly revised and updated by noted experts. These updates address the modernization of clinical virology and new developments in the field.

Contents:

Contributors
Author Biographies
Preface to the Fifth Edition
SECTION I
GENERAL TOPICS IN CLINICAL VIROLOGY
1 The Taxonomy, Classification, and Characterization of Medically Important Viruses / 3
STEVEN J. DREWS
2 Quality Assurance and Quality Control in Clinical and Molecular Virology / 27
MATTHEW J. BANKOWSKI
3 Regulatory Compliance / 35
LINOJ SAMUEL
4 Laboratory Safety / 41
K. SUE KEHL
5 Laboratory Design / 51
MATTHEW J. BINNICKER
SECTION II
LABORATORY PROCEDURES FOR DETECTING VIRUSES
6 Specimen Requirements Selection, Collection, Transport, and Processing / 59
REETI KHARE AND THOMAS E. GRYS
7 Primary Isolation of Viruses / 79
MARIE L. LANDRY AND DIANE LELAND
8 Viral Antigen Detection / 95
DIANE S. LELAND AND RYAN F. RELICH
9 Serologic (Antibody Detection)
Methods / 105
DONGXIANG XIA, DEBRA A. WADFORD,
CHRISTOPHER P. PREAS, AND DAVID P. SCHNURR
10 Nucleic Acid Extraction in Diagnostic Virology / 117
RAYMOND H. WIDEN AND SUZANE SILBERT
11 Nucleic Acid Amplification by Polymerase Chain Reaction / 129
ANA MARIA CARDENAS AND KEVIN ALBY
12 Isothermal Nucleic Acid Amplification Methods / 137
HARALD H. KESSLER AND EVELYN STELZL
13 Quantitative Molecular Methods / 145
NATALIE N. WHITFIELD AND DONNA M. WOLK
14 Signal Amplification Methods / 167
YUN (WAYNE) WANG
15 DNA Sequencing for Clinical and Public Health Virology: Some Assembly Required / 173
JOANNE BARTKUS
16 Phenotypic and Genotypic Antiviral Susceptibility Testing / 201
MARTHA T. VAN DER BEEK AND ERIC C. J. CLAAS
17 Point-of-Care Diagnostic Virology / 229
JAMES J. DUNN AND LAKSHMI CHANDRAMOHAN
18 Future Technology / 243
ERIN MCELVANIA TEKIPPE AND CAREY-ANN D. BURNHAM
SECTION III
VIRAL PATHOGENS
19 Respiratory Viruses / 257
CHRISTINE ROBINSON, MICHAEL J. LOEFFELHOLZ,
AND BENJAMIN A. PINSKY
20 Enteroviruses and Parechoviruses / 277
M. STEVEN OBERSTE AND MARK A. PALLANSCH
21 Measles, Mumps, and Rubella Viruses / 293
WILLIAM J. BELLINI, JOSEPH P. ICENOGLE, AND CAROLE J. HICKMAN
22 Gastrointestinal Viruses / 311
MICHAEL D. BOWEN
23 Hepatitis A and E Viruses / 329
GILBERTO VAUGHAN AND MICHAEL A. PURDY
24 Hepatitis B and D Viruses / 341
REBECCA T. HORVAT
25 Hepatitis C Virus / 351
MELANIE MALLORY AND DAVID HILLYARD
26 Herpes Simplex Viruses and Varicella Zoster Virus / 363
SCOTT H. JAMES AND MARK N. PRICHARD
27 Cytomegalovirus / 373
PREETI PANCHOLI AND STANLEY I. MARTIN
28 Epstein-Barr Virus / 387
DERRICK CHEN AND BELINDA YEN-LIEBERMAN
29 Human Herpesviruses 6, 7, and 8 / 399
SHEILA C. DOLLARD AND TIMOTHY M. KARNAUCHOW
30 Human Papillomaviruses / 413
SUSAN NOVAK-WEEKLEY AND ROBERT PRETORIUS
31 Human Polyomaviruses / 427
REBECCA J. ROCKETT, MICHAEL D. NISSEN, THEO
P. SLOOTS, AND SEWERYN BIALASIEWICZ
32 Parvoviruses / 443
RICHARD S. BULLER
33 Poxviruses / 457
ASHLEY V. KONDAS AND VICTORIA A. OLSON
34 Rabies Virus / 473
ROBERT J. RUDD AND APRIL D. DAVIS
35 Arboviruses / 493
LAURA D. KRAMER, ELIZABETH B. KAUFFMAN,
NORMA P. TAVAKOLI
36 Animal-Borne Viruses / 515
GREGORY J. BERRY, MICHAEL J. LOEFFELHOLZ, AND GUSTAVO PALACIOS
37 Human Immunodeficiency Viruses and
Human T-lymphotropic Viruses / 527
JOERG SCHUEPBACH
38 Chlamydiae / 545
BARBARAVAN DER POL AND CHARLOTTE A. GAYDOS
39 The Human Virome / 561
MATTHEW C. ROSS, NADIM J. AJAMI, AND JOSEPH F. PETROSINO
40 Human Susceptibility and Response
to Viral Diseases / 567
VILLE PELTOLA AND JORMA ILONEN
APPENDIXES: REFERENCE VIROLOGY
LABORATORIES
APPENDIX 1 Reference Virology Laboratory
Testing Performed at the Centers for Disease Control / 581
ROBERTA B. CAREY
APPENDIX 2 Public Health Laboratory Virology Services / 585
JANE GETCHELL
APPENDIX 3 International Reference Laboratories Offering Virology Services / 595
ARIEL I. SUAREZ AND CRISTINA VIDELA
INDEX / 607