Main description:

This detailed volume provides various cellular and experimental animal models and research methodologies to understand the complex interrelated pathophysiological mechanisms triggered by hyperglycemia that underlie the development of diabetic retinopathy. The book covers methods for understanding the pathogenesis of diabetic retinopathy, detection and screening of biomarkers in diabetic retinopathy, as well as novel technologies for diabetic retinopathy management. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Diabetic Retinopathy: Methods and Protocols serves as an invaluable resource for scientists and clinicians who are interested in the area of ophthalmology research.

Contents:

1. Induction of Type 2 Diabetes in Mice to Understand Vascular Changes that Drive Diabetic Retinopathy

Emily Attrill, Stephen M. Richards, Renee M. Ross, Brad A. Sutherland, and Dino Premilovac

2. Akimba Proliferative Diabetic Retinopathy Model: Understanding Molecular Mechanism and Drug Screening for the Progression of Diabetic Retinopathy

Sai Bo Bo Tun and Veluchamy Amutha Barathi

3. Oxygen-Induced Retinopathy in the Rat: An Animal Model to Study the Proliferative Retinal Vascular Pathology

Yu-Fan Chuang and Fan-Li Lin

4. Retinal Assessment Using In Vivo Electroretinography and Optical Coherence Tomography in Rodent Models of Diabetes

Da Zhao, Pei Ying Lee, Vickie H.Y. Wong, Tomoharu Nishimura, Anh Hoang, Katie K.N. Tran, Anna K. van Koeverden, Brianna C. Afiat, Christine T.O. Nguyen, and Bang V. Bui

5. A Guide to Genome Wide Association Study Design for Diabetic Retinopathy

Rajya L. Gurung, Kathryn P. Burdon, and Bennet J. McComish

6. Single Cell RNA Sequencing (scRNA-Seq) Data Analysis of Retinal Homeostasis and Degeneration of Microglia

Madhu Sudhana Saddala, Srilatha Mundla, Naina Patyal, and Srujanika Dash

7. Bioinformatics Tools for Bulk Gene Expression Deconvolution in Diabetic Retinopathy

Ru Qi Teh, Guei-Sheung Liu, and Jiang-Hui Wang

8. MicroRNA Profiling from Tears as a Potential Non-Invasive Method for Early Detection of Diabetes Retinopathy

Wilson K. M. Wong, Mya Sara, Ryan J. Farr, Pooja S. Kunte, Hrishikesh P. Hardikar, Chittaranjan S. Yajnik, Anandwardhan A. Hardikar, and Mugdha V. Joglekar

9. Proximity Extension Assay (PEA) Platform to Detect Vitreous Biomarkers of Diabetic Retinopathy

Ricardo Lamy, Showgy Ma'ayeh, Sarantis Chlamydas, and Jay M. Stewart

10. Methods for Analyzing the Gut Microbiota in Diabetic Retinopathy

Yinhua Huang, Jacey Hongjie Ma, Yanfang Peng, Xu Yang, Jiansu Chen, and Shibo Tang

11. Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline

Sarah Weber, Nick Carruthers, Chris Gates, Yuanjun Zhao, and Jeffrey Sundstrom

12. Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants

Satheesh Kumar, Jiang-Hui Wang, and Guei-Sheung Liu

13. Methods for RPE Sheet Construction Using iPS-CM in Conjunction with Natural Scaffold of Corneal Lenticule Derived from SMILE

Jianing Gu, Yini Wang, Chengcheng Ding, Shibo Tang, and Jiansu Chen

14. Chromatin Immunoprecipitation Assay in Mouse Retinal Tissue

Zhijie Wang, Shangli Ji, Jacey Hongjie Ma, Jiansu Chen, and Shibo Tang

15. Frozen Block Tissue Staining for Eye Structure of Zebrafish Embryo

Yujin Lee and Jaewook Yang

16. Investigating the Function of MicroRNAs in Human Retinal Microvascular Endothelial Cells of Diabetic Retinopathy

Yong Zeng, Zekai Cui, Jiansu Chen, and Shibo Tang

17. Genome Editing Inhibits Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy

Wenyi Wu and Hetian Lei