Main description:

This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful tool, examples of where the ELISA or similar immunoassay formats are currently being used, and newer techniques that may have a significant impact on future applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, ELISA: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.

Contents:

1. Enzyme-linked Immunosorbent Assay: Types and Applications

Hovhannes Hayrapetyan, Thao Tran, Eglis Tellez-Corrales, and Charitha Madiraju

2. ELISA Essentials: surfaces, antibodies, enzymes and substrates

Robert S. Matson

3. Antibody Immobilization

Robert S. Matson

4. Screening for Antibody Specificity and Sensitivity with ELISA

Michael J. Halat and Emily Gravens

5. Effective blocking and stabilizing methods using synthetic polymer on ELISA

Tomozuml Noda, Masaru Matsuda, Hirotaka Suzuki, Yusuke Okaswa, and Motohiro Mitani

6. Gas plasma surface modification for biological assays

Khoren Sahagian

7. Interference in ELISA

Robert S. Matson

8. Determination of Proinflammatory And Anti-Inflammatory Cytokines By Elisa Technique

Osmany Blanco Munoz

9. Gyrolab immunoassays - Miniaturization, automation and integration into a rapid workflow

Johan Engstrom

10. Assessment of Immunologically Potent Carbohydrates

Denong Wang

11. Lateral Flow Microarray based ELISA for Cytokines

Robert S. Matson

12. A unique multiplex ELISA to profile growth factors and cytokines in cerebrospinal fluid

Charitha Madiraju, Amani Sastry, Martha Oppong, Jacob Karp, Maryla Krajewska, Stan Krajewski, Barbara Tomik, Andrzej Szczudlik, and Robert Matson

13. Well-based Multiplex Food Allergen Colorimetric ELISA

Robert S. Matson

14. Mycotoxin Quantification by Competitive ELISA

Bryn E Merrill and Robert S Matson

15. Lumit: A Homogeneous Bioluminescent Immunoassay for Detecting Diverse Analytes and Intracellular Protein targets

Byounghoon (Brian) Hwang, Juliano Alves, Dan Lazar, Nidhi Nath, Laurie Engel, Martha O'Brien, Kevin Hsiao, Kevin Kupcho, Becky Godat, Rod Flemming, Said Goueli, and Hicham Zegzouti

16. ELISA-based Biosensors

Robert S. Matson