Main description:

This book compiles the relevant information related to genome editing tools and their roles in crop improvement. The book contains a brief introduction about various genome editing tools and their application in major crops. It discusses the genome editing approaches and the strategies used for genome editing in different crops. Some of the chapters cover the detailed methodology of sgRNA design, vector construction and transformation in different crops followed by data analysis. A few chapters focus on the applications of genome editing tools towards crop improvement. This book will be of particular interest to plant biologists working in the field of genome editing and crop breeders. It will provide valuable information and useful material for our readers' experimental work.

Contents:

PART I: Introduction to genome editing and Crop improvementChapter 1: Genome editing is a recently emerged advanced technologyAuthors: Rukmini Mishra, Raj Kumar Joshi, Kaijun ZhaoSummary: Briefly describe the history of genome editing technology, mentioning Mega nuclease, ZFNs, TALENs, and CRISPR/Cas systems.Chapter 2: Genome editing is revolutionizing crop improvement and agricultureAuthors: Raj Kumar Joshi, Rukmini Mishra, Kaijun ZhaoSummary: Particular description about the CRISPR/Cas-based genome editing tools and the base editor systems for highly efficient crop improvement in future.PART II: CRISPR Genome editing approachesChapter 3: Genome editing with CRISPR/Cas9 RNA-directed nuclease.Authors: Akshaya Biswal, University of North Carolina, Chapel hill, USAChapter 4: CRISPR/Cas9 based multi gene editing system.Authors: Prof. Yinong Yang, Pennslyvania State University, USA.Chapter 5: Genome editing using a single transcript CRISPR/Cas9 system.Authors: Prof. Daniel F Voytas, University of Minnesota, USA.Chapter 6: Genome editing using the CRISPR/Cpf1 system.Authors: Anindya Bandopadhyay, Syngenta Pvt. Ltd, INDIAChapter 7: Genome editing using Cas9/gRNA Ribonucleoproteins (RNPs).Authors: Sergei Svitashev, DuPont Pioneer, Johnston, Iowa, USA.Chapter 8: Induction of genetic variation using programmable base editors.Authors: Zenpei Shimatani, Kobe, JapanPART III: Genome editing strategies-NHEL and HR, and base-editingChapter 9: CRISPR/Cas9 assisted non-homologous end joining strategy for engineering plant genome.Authors: Suhas B Sutar, Xia Lanqin, CAAS, China.Chapter 10: A dual sgRNA/cas9 design for targeted gene replacement in plants.Authors: Dr Yiping Qi- University of Maryland, USChapter 11: Gene targeting by homology-directed repair using a geminivirus-based CRISPR/Cas9 System.Authors: Jian-Kan Zhu & Group, Perdue University, USAChapter 12: Homologous recombination mediated plant genome editing using guide RNA/cas9.Authors: Rukmini Mishra and Kaijun Zhao, Beijing, ChinaChapter 13: Improved base editor for efficiently inducing genetic variations with CRISPR/Cas9-guided hyperactive hAID mutant.Authors: Beum Chang Kang, Daejeon, South KoreaPART D: Vector construction for plant genome editingChapter 14: Development of a CRISPR/Cas9 tool kit for sgRNA delivery and genome editing.Authors: Baohong Zhang & Group, East Carolina University, USAChapter 15: Development of single step assembly CRISPR/Cas9 vectors for multiple gRNA expression.Authors: Prof. Yao-Guang Liu & Group, South China Agricultural University, ChinaChapter 16: Designing of CRISPR/Cpf1 system and its assessment for genome editing in plants.Authors: Prof. Caxia Gao & Group, Institute of Genetics and Developmental Biology, CAS, ChinaChapter 17: DNA free genome editing in crops using CRISPR/Cpf1 system.Authors: Hyeran Kim & Group, University of Koln, Germany.Chapter 18: Development of an optimized vector system for precision base editing using Cas9-cytidine/adenosine deaminase fusion.Authors: Huanbin Zhou & Group, Institute of Plant Protection, CAAS, China.Part E: Genome editing towards crop improvementSummary: Briefly describe the current applications of genome editing tools in genetic improvement of different crops for agronomically important traits such as yield, quality and disease resistance.Chapter 19: Genome editing for rice improvementAuthors: Prof. Kaijun Zhao (CAAS, China) and Bing Yang (Iowa State University, USA).Chapter 20: Genome editing for wheat improvement.Authors: Vladimir Nekrasov, Rothamsted Research, UK.Chapter 21: Genome editing for maize improvement.Authors: Jinrui Shi, Jeffery Habben & group, DuPont Pioneer, USAChapter 22: Genome editing for barley improvement.Authors: Wendy Harwood & Group, John Innes Centre, UKChapter 23: Genome editing for tomato improvement.Authors: Adwaita Parida, Arun K. Sharma, University of Delhi, IndiaChapter 24: Genome editing for potato improvement.Authors: David Douches & Group, Michigan State University, Michigan, USAChapter 25: Genome editing for chilli improvement.Authors: Rukmini Mishra, Raj Kumar Joshi, RDWU, INDIAChapter 26: Genome editing for citrus improvement.Authors: Jaffery Jones, Nian Wang & Group, University of Florida, USAChapter 27: Genome editing for cotton improvementAuthors: Chunpeng Song & Group, Henan University, China.Chapter 28: Genome editing for improvement of oil seed crops.Authors: Swati Megha, Raj Kumar Joshi, Nat NV Kav, University of Alberta, CanadaChapter 29: Genome editing of other cropsAuthors: Prof. Kaijun Zhao & Group, Institute of Crop Sciences, CAAS, China.