Main description:

This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it.

Contents:

Part I: Overview

1. The Factory of Blood Production: Hematopoietic Stem Cells

Jonathan Hoggatt and Louis M. Pelus

Part II: Molecular Modification and Evaluation

2. Detection of DNA Damage in Hematopoietic Stem Cells

Saipriya Ayyar and Isabel Beerman

3. Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation

Liqiong Liu

4. CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells

Reza Shahbazi, Patricia Lipson, Karthikeya S.V. Gottimukkala, Daniel D. Lane, and Jennifer E. Adair

5. Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Chuanfeng Wu, So Gun Hong, Aylin Bonifacino, and Cynthia E. Dunbar

Part III: Cellular Isolation and Characterization

6. Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Stefan Radtke and Hans-Peter Kiem

7. Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells

Diego A. Lopez and Anna E. Beaudin

8. Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype

Safa F. Mohamad and Maegan L. Capitano

9. Hematopoietic Stem Cell Identification Post-Radiation

Andrea M. Patterson, Christie M. Orschell, and Louis M. Pelus

Part IV: The Hematopoietic Niche and Accessory Cells

10. Intravital Microscopy for Hematopoietic Studies

Myriam Haltalli and Cristina Lo Celso

11. Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology

Christa Haase, Dmitry Richter, and Charles P. Lin

12. MSC and HSPC Co-Culture: Mimicking Ex Vivo Bone Marrow Niche

Pratibha Singh

13. Isolation of Thymus Stromal Cells from Human and Murine Tissue

Karin Gustafsson and David T. Scadden

Part V: Transplantation and In Vivo Models

14. Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation

Scott H. Cooper, Maegan L. Capitano, and Hal E. Broxmeyer

15. Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish

Ellen Fraint, Peng Lv, Feng Liu, Teresa V. Bowman, and Owen J. Tamplin

16. Establishing a Murine Model of the Hematopoietic Acute Radiation Syndrome

P. Artur Plett, Louis M. Pelus, and Christie M. Orschell

17. Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells

Malwina Suszynska, Mateusz Adamiak, Arjun Thapa, Monika Cymer, Janina Ratajczak, Magdalena Kucia, and Mariusz Z. Ratajczak