Main description:

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.

Contents:

Dedication...Preface...Table of Contents...Contributing Authors...
1. Human Monocolonal Antibodies: The Benefits of HumanizationHerman Waldmann
2. Cancer Immunotherapy: The Dawn of Antibody CocktailsIlaria Marrocco, Donatella Romaniello, and Yosef Yarden
3. IgM Natural Autoantibodies in Physiology and the Treatment of DiseaseMahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, and Moses Rodriguez
4. Monoclonal Antibodies for the Treatment of Melanoma: Present and Future StrategiesMadhuri Bhandaru and Anand Rotte
5. An Efficient Method to generate Monoclonal Antibodies from Human B CellsJenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, and Patrick C. Wilson
6. Isolation of Antigen-Specific, antibody-Secreting Cells using a Chip-Based Immunospot ArrayHiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, and Atsushi Muraguchi
7. Purification of Human Monoclonal antibodies and Their FragmentsNicole Ulmer, Sebastian Vigg, Thomas Muller-Spath, and Massimo Morbidelli
8. One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral BloodAnna-Jasmina Donaubauer, Paul F. Ruhle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, and Benjamin Frey
9. Humanization and Simultaneous Optimization of Monoclonal AntibodyT. Kuramochi, T. Igawa, H. Tsunoda, and K. Hattori
10. Antibody Fragments Humanization: Beginning with the End in MindNicolas Aubrey and Philippe Billiald
11. Antigen-Specific Human Monoclonal Antibodies from Transgenic MiceSusana Magadan Mompo and Africa Gonzalez-Fernandez
12. Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab FrameworkJeremy D. King and John C. Williams
13. Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen ReceptorsKeichiro Mihara, Tetsumi Yoshida, and Joyeeta Bhattacharyya
14. Rapid Chimerization of AntibodiesKoji Hashimoto, Kohei Kurosawa, Hidetaka Seo, and Kunihiro Ohta
15. Phage Display Technology for Human Monoclonal AntibodiesDal Ferro Marco, Rizzo Serena, Rizzo Emanuela, Marano Francesca, Luisis Immacoloata, Tarasiuk Olga, and Daniele Sblattero
16. Recombinant Antibody Selections by Combining Phage and Yeast DisplayFortunato Ferrara, Maria Felicia Soluri, and Daniele Sblattero
17. Epitope Mapping via Phage Display from Single Gene LibrariesViola Fuhner, Philip Alexander Heine, Kilian Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marcal Schmidt Garcia Moreira, Michael Hust, and Giulio Russo
18. A High-Throughput Magnetic Nanoparticle Based Semi-Automated Antibody Phage Display BiopanningAngela Chiew Wen Ch'ng, Azimah Ahmad, Zoltan Konthur, and Theam Soon Lim
19. A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin Ivan Babic, Santosh Kesari, and Mark C. Glossy
20. A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan ReceptorsPatricia Blundell and Richard Pleass
21. A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors Induced by Therapeutic Monoclonal AntibodiesMichihiko Aoyama, Minoru Tada, and Akiko Ishii-Watabe
22. "BIClonals": Production of Bispecific Antibodies in IgG Format in Transiently Transfected Mammalian CellsDana Litvak-Greenfeld, Lilach Vaks, Stav Dror, Limor Nahary, and Itai Benhar
23. Production of Stabilized Antibody Fragments in the E. coli Bacterial Cytoplasm and in Transiently-Transfected Mammalian CellsRacheli Birnboim-Perach, Yehudit Grinberg, Lilach Vaks, Limor Nahary, and Itai Benhar