Main description:

This volume explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances. The chapters in this book cover methods for studying receptor-ligand interactions at both molecular and cellular levels; methods to create and characterize novel antibody reagents; and methods to characterize the molecular processes that lead to adaptive receptor maturation. This book also contains chapters that look at high-throughput strategies that describe the diversity of immune receptors and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Immune Receptors: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers interested in learning more about this developing field.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc ReceptorsJordan D. Mohr, Meraj Ramezani, David Holowka, and Barbara A. Baird
2. Low-Cost and Cell-Surface-Mimic Analysis of Ligand Interactions of Biotinylated Immune Receptors Using Surface Plasmon Resonance Kimiko Kuroki, Hideo Fukuhara, Takashi Tadokoro, and Katsumi Maenaka
3. Analysis of the Sub-Cellular Localization of NLRs Upon Shigella flexneri Infection Exemplified by NOD1Christine Arnold, Kornelia Ellwanger, and Thomas A. Kufer
4. Generation of VNAR Libraries from Immunized Sharks and Selection of Target-Specific Clones Helen Dooley
5. Identification of Glycan-Specific Variable Lymphocyte Receptors using Yeast Surface Display and Glycan Microarrays Tanya R. McKitrick, Melinda S. Hanes, Charles S. Rosenberg, Jamie Heimburg-Molinaro, Max D. Cooper, Brantley R. Herrin, and Richard D. Cummings
6. Cell Aggregation Assays for Homophilic Interactions between Cell Surface ProteinAidan L. Huene and Matthew L. Nicotra
7. Measuring Mutator Enzyme Activity using an E. coli-Based Colony Formation AssayMei-Chen Liu and Sebastian D. Fugmann
8. Lamprey Variable Lymphocyte Receptor Monoclonal Antibodies for Whole Cell Surface AntigensGayathri Bommakanti
9. A Role for Secreted Immune Effectors in Microbial Biofilm Formation Revealed by Simple In Vitro Assays Assunta Liberti, Brittany A. Leigh, Zachary Graham, and Larry J. Dishaw
10. Assessment of Scavenger Receptor Cysteine Rich Domain Binding to Bacteria Janice C. Telfer, Haoting Hsu, Miles Dae Tyner, and Lauren Le Page
11. Bioinformatics Approaches for Analyzing Multigene Families Encoding Immune ReceptorsKatherine M. Buckley
12. Production and Characterization of SIV-Specific CAR/CXCR5 T CellsMary S. Pampusch, Agnes Hajduczki, Gwantwa Mwakalundwa, Elizabeth Connick, Edward A. Berger, and Pamela J. Skinner
13. Engineering IgG1 Fc Domains that Activate the Complement System Chang-Han Lee and George Delidakis
14. An Imaging Flow Cytometry Protocol for Studying Immunoregulatory Receptor-Mediated Regulation of Phagocytosis Myron A. Zwozdesky, Chenjie Fei, and James L. Stafford
15. Single Cell Sequencing for EverybodyValentina Proserpio, Vanessa Falvo, Carlotta Duval, Giacomo Donati, and Salvatore Oliviero
16. Exploring the Diversity of the B-Cell Receptor Repertoire Through High-Throughput SequencingJennifer R. Hom, Deepak Tomar, and Christopher M. Tipton
17. Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell StudiesJohanna S. Selvaratnam, Tracy S.H. In, and Michele K. Anderson
Subject Index List...