Main description:

This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the theory and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field

Contents:

1. Introduction to Immunohistochemistry. From Evolving Science to Timeless Art

Luis Del Valle

2. Immunohistochemistry in Historical Perspective. Knowing the Past to Understand the Present

Carlos Ortiz-Hidalgo

3. Sample Preparation & Fixation for Histology and Pathology

Enrique Agustin Sampedro Carrillo

4. Tissue Processing

Sarah J. Aziz, and Cady E. Zeman-Pocrnich

5. Antigen Retrieval and Signal Amplification

Logan Dunkenberger, and Luis Del Valle

6. Primary Antibody Selection and Blocking Techniques for Immunohistochemistry

Lori A. Peton, and Miguel Reyes-Mugica

7. Probes for Fluorescent Visualization of Specific Cellular Organelles

Timothy Paul Foster

8. Enzymatic Immunohistochemistry

Rodolfo Henrich Lobo

9. Fluorescent Immunohistochemistry

Vanessa Moreno, Elizabeth A. Smith, and Sergio Pina-Oviedo

10. Double Labeling Fluorescent Immunocytochemistry

Monika Rak, and Krzysztof Reiss

11. Multiplexing and Spectral Microscopy

Logan Dunkenberger, Adriana Zapata, and Luis Del Valle

12. Fluorescent In situ Hybridization (FISH)

Jeanette M. Ramos

13. Proximity Ligation Assay (PLA)

Muhammad S. Alam

14. Immunohistochemistry, Quality control and Principles of Validation in the Central Nervous System

Veronica Velasco-Vales, Danny Soria-Cespedes, Teresa Cristina Cuesta-Mejias, and Norma Cecilia Padron-Perez

15. Culture and Phenotyping of Glial Cell Cultures, Gliospheres and Neurospheres.

Amanda Parker-Struckhoff, Krzysztof Reiss, and Luis Del Valle

16. ER+ Breast Cancer Mammosphere Formation and Analysis

Ciera S. Singleton, Leo Li-Ying Chan, Kelsey J. McCulley, Sarah L. Kessel, Luis Del Valle, and Judy S. Crabtree

17. Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSCs) by Flow Cytometry

Maria Dulfary Sanchez-Pino

18. Comet Assay for the Detection of Single and Double Strand DNA Breaks

Logan Dunkenberger, Krzysztof Reiss, and Luis Del Valle

19. Fluorescent-Activated Cell Sorting (Flow Cytometry)

Luis F. Carrillo