Main description:

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.

Contents:

Preface...Table of Contents...Contributing Authors...
1. High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical GuidebookFlorian Mair and Aaron J. Tyznik
2. Immunophenotyping by Mass CytometryGregory K. Behbehani
3. Fluorescent Cell Barcoding for ImmunophenotypingValentina Giudice. Giovanna Fantoni, and Angelique Biancotto
4. Immunophenotyping using Dried and Lyophilized ReagentsMarc Langweiler
5. Guidelines for Gating Flow Cytometry Data for Immunological AssaysJanet Staats, Anagha Divekar, J. Philip McCoy, and Holden T. Maecker
6. Strategies and Techniques for NK Cell PhenotypingChen Ziqing, Andreas Lundqvist, and Kristina Witt
7. Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose TissueAlain Diaz, Maria Romero, Daniela Frasca, and Bonnie B. Blomberg
8. Multiparametric Flow Cytometry Analysis of Naive, Memory, and Effector T CellsAnkit Saxena, Pradeep K. Dagur, and Angelique Biancotto
9. Immunophenotyping of Human Regulatory T CellsJanet Staats
10. Immunophenotyping of Human Innate Lymphoid CellsSara Trabanelli, Alejandra Gomez-Cadena, and Camilla Jandus
11. Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric AnalysisAbdullah Alsuwaidan, Franklin Fuda, Weina Chen, and Mingyi Chen
12. Immunophenotyping of Circulating Endothelial Cell and Endothelial MicroparticlesNicholas Wanner and Kewal Asosingh
13. Rare Event Phenotyping and Molecular Characterization: Circulating Tumor CellsMoen Sen, Ling Wang, Liping Yu, and Erica L. Carpenter
14. Quality Control of ImmunophenotypingBruce Greig
15. Immunophenotyping of Acute Myeloid LeukemiaPallavi Kanwar Galera, Chunjie Jiang, and Raul Braylan
16. Immunophenotyping of Acute Lymphoblastic LeukemiaJoseph A. DiGiuseppe and Jolene L. Cadinali
17. Clinical Flow Cytometry Testing in Chronic Lymphocytic LeukemiaDalia A. Salem and Maryalice Stetler-Stevenson
18. Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)Andrea J. Illingworth, Iuri Marinov, and D. Robert Sutherland