Main description:

This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Introduction to the Immune SystemScott McComb, Aude Thiriot, Bassel Akache, Lakshmi Krishnan, and Felicity Stark
2. Immunoproteomics Methods and TechniquesKelly M. Fulton, Isabel Baltat, and Susan M. Twine
3. Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen IdentificationKelly M. Fulton, Anna Ananchenko, Lawrence Wolfraim, Shannon Martin, and Susan M. Twine
4. Profiling of Cytokine and Chemokine Responses using Multiplex Bead Array TechnologyGreg Harris and Wangxue Chen
5. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry AnalysisTimothy D. Veenstra
6. Enriching for Low Abundance Serum Proteins using ProteoMiner (TM) and Protein Level HPLCSophie Moggridge, Kelly M. Fulton, and Susan M. Twine
7. Identification of the Antigen Content of ElectroimmunoprecipitatesN. Helena Beyer and Niels H. H. Heegaard
8. Detection of Human Norovirus-Specific Antibodies using the Luciferase Immunoprecipitation System (LIPS) AssayChristine M. Tin and Stanislav V. Sosnovtsev
9. Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from SerumChristie E. Delaney, John F. Kelly, Wen Ding, and Arsalan S. Haqqani
10. Antigen Identification for Cell-Binding Antibodies using Ligand Directed Crosslinking and Biotin TransferTammy-Lynn Tremblay and Jennifer J. Hill
11. Whole Genome-Phage Display Libraries: A Powerful Tool for Antigen DiscoveryElisa Beghetto and Nicola Gargano
12. Multiplexed Detection of Autoantibodies to Glycopeptides using MicroarrayJohannes W. Pedersen, Alexander Nostdal, and Hans H. Wandall
13. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope IdentificationFei Wen, Mason R. Smith, and Huimin Zhao
14. Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity PurificationSarah A. Penny and Stacy A. Malaker
15. Enhanced Mass Spectrometry Detection of MHC PeptidesRui Chen and Jianjun Li
16. Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide ParticlesRui Chen and Jianjun Li
17. Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing PathwaysStacy A. Malaker and Michael J. Ferracane
18. Genome Based Bioinformatic Prediction of Major Histocompatibility (MHC)Simon J. Foote
19. Enhancing Mass Spectrometry-Based MHC-I Peptide Identification through a Targeted Database Search ApproachPrathyusha Konda, J. Patrick Murphy, Morten Nielsen, and Shashi Gujar
20. Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell InteractomicsArsalan S. Haqqani and Danica B. Stanimirovic
21. Genomics Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos, Volker Stadler, and David K. Han
22. Identification of Antigens in Immune ComplexesNozomi Aibara and Kaname Ohyama
23. Analysis and Characterization of Immune Cells and their Activation Status by Whole-Cell MALDI TOF Mass SpectrometryRichard Ouedraogo, Julien Textoris, Laurent Gorvel, Aurelie Daumas, Christian Capo, and Jean-Louis Mege
24. A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of InfluenzaKevin M. Downard
25. Immunoproteomic Biomarkers: From Publication to Personalized MedicineDevanand Pinto