Main description:

This volume provides a broad spectrum of research models, techniques, and protocols. Chapters are divided into sections providing detailed information on the background and context for the chosen topic of interest, a list of the necessary materials and reagents needed for each topic, as well as the step-by-step laboratory protocols and methodologies for the successful and reproducible execution of each topic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Inflammation and Cancer: Methods and Protocols aims to provide information to students and researchers at all levels of experience for further study into this vital field.

Contents:

Part I: Experimental Model Systems

1. In Vivo Models of Inflammatory Bowel Disease and Colitis-Associated Cancer

Ka Yee Fung and Tracy Putoczki

2 Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer

Sarah A. Best, Ariena Kersbergen, Marie-Liesse Asselin-Labat, and Kate D. Sutherland

3. Utility of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Preclinical Evaluation of Therapies in Cancer

William Berry and Daniel Croagh

4. Modelling Intestinal Carcinogenesis Using In Vitro Organoid
Cultures

Thierry Jarde, Genevieve Kerr, Reyhan Akhtar, and Helen Abud

5. Protocols to Evaluate Cigarette Smoke-induced Lung Inflammation

and Pathology in Mice

Ross Vlahos and Steven Bozinovski

6. Tracking Competent Host Defence to Chronic Inflammation: An In vivo Model of Peritonitis Javier U. Fernandez, David Millrine, and Simon A. Jones

7. Citrobacter rodentium Infection Model for the Analysis of Bacterial Pathogenesis and Mucosal Immunology

Catherine L. Kennedy and Elizabeth L. Hartland

8. In Vivo Infection Model of Severe Influenza a Virus

Ashley Mansell and Michelle D. Tate

9. In Vivo Models for Inflammatory Arthritis Gareth W. Jones, David G. Hill, Katie Sime, and Anwen S. Williams

10. Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease

Le Son Tran and Richard L. Ferrero

11. Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer
Christoph Garbers and Stefan Rose-John

Part II: Experimental Techniques

12. Laser Microdissection of Cellular Compartments for Expression Analyses in Cancer Models

Tae-Su Han and Masanobu Oshima

13. Tissue Processing for Stereological Analyses of Lung Structure in Chronic Obstructive Pulmonary Disease

Mohamed Saad and Saleela M. Ruwanpura

14. Quantifying Caspase-1 Activity in Murine Macrophages
Dave Boucher, Amy Chan, Connie Ross, and Kate Schroder

15. Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation

Benjamin J. Shields, Andrew Keniry, Marnie E. Blewitt, and Matthew P. McCormack

16. Analysis of DNA Methylation in Tissues Exposed to Inflammation

Naoko Hattori and Toshikazu Ushijima

17. A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens

Leonie A Cluse, Iva Nikolic, Deborah Knight, Piyush B Madhamshettiwar, Jennii Luu, Karla J Cowley, Timothy Semple, Gisela Mir Arnau, Jake Shortt, Ricky W Johnstone, and Kaylene J Simpson

18. Immuno-detection of Immature and Bioactive Forms of the Inflammatory Cytokine IL-18 Brendan J. Jenkins and Virginie Deswaerte

19. Optimisation Techniques for miRNA Expression in Low Frequency Immune Cell Populations

Victoria G. Lyons, Natalie L. Payne, and Claire E. McCoy

20. Assessing the cGAS-cGAMP-STING Activity of Cancer Cells

Genevieve Pepin and Michael Gantier

21. Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies Nicholas P.D. Liau and Jeffrey J. Babon

22. Production of Recombinant Killer Immunoglobulin-like Receptors for Crystallography and Luminex Based Assays

Phillip Pymm and Julian P. Vivian

23. Isolation and Characterisation of Mouse Intrahepatic Lymphocytes by Flow Cytometry

Florian Wiede and Tony Tiganis