Main description:

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease.

Contents:

Preface...Table of Contents...Contributing Authors...
1. Studying the Pro-Migratory Effects of MIFAdrian Hoffmann, Leon Christian Zwissler, Omar El Bounkari, and Jurgen Bernhagen
2. In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions under Flow ConditionsMichael J. Hickey
3. Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In VivoM. Ursula Norman and Michael J. Hickey
4. Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune CellsTali Lang
5. Measuring MIF in Biological FluidsFabien B. Vincent and Tali Lang
6. Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wildtype and MIF KO Mice Jacqueline K. Flynn, Nadia Deen, and James Harris
7. Genotyping Two Promoter Polymorphisms in the MIF Gene: a -794 CATT5-8 Microsatellite Repeat and a -173 G/C SNPLin Leng, Edwin Siu, and Richard Bucala
8. Staining MIF in Cells for Confocal MicroscopyJames Harris
9. Microscopy Methods for Imaging MIF and its Interaction PartnersKirstin D. Elgass, Sarah J. Creed, and Ina Rudloff
10. Co-Immunoprecipitation (Co-IP) of Macrophage Migration Inhibitory Factor (MIF)Jawad H. Abidi, James Harris, and Nadia S. Deen
11. Concurrent Immunohistochemical Localization and Western Blot Analysis of the MIF Receptor, CD74, in Formalin-Fixed, Paraffin-Embedded TissueAmanda Graham and Warren B. Nothnick
12. Methods to Determine the Effects of MIF on In Vitro Osteoclastogenesis using Murine Bone Marrow-Derived Cells and Human Peripheral Blood Mononuclear Cells (PBMCs)Ran Gu
13. Inducing and Inhibiting Autophagy to Investigate its Interactions with MIFNadia S. Deen, Jacinta P. Lee, and James Harris
14. Assays for Measuring the Role of MIF in NLRP3 Inflammasome ActivationAnita Pinar and James Harris
15. Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release Shahrzad Zamani, Eric F. Morand, and Jacqueline K. Flynn
16. Assessing the Role of MIF in Plasmodium spp. Infections using Ex Vivo ModelsElizabeth H. Aitken
17. The Effect of Macrophage Migration Inhibitory Factor on Intestinal Permeability: FITC-Dextran Serum Measurement and Transmission Electron Microscopy Milica Vujicic, Sanja Despotovic, Tamara Saksida, and Ivana Stojanovic
18. Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic AsthmaVenkata Sita Rama Raju Allam and Maria B. Sukkar
19. Investigating MIF in Mouse Model of GoutIzabela Galvao, Allysson Cramer, and Flavio Almeida Amaral
20. 5and 3' RACE Method to Obtain Full-Length 5' and 3' Ends of Ciona robusta Macrophage Migration Inhibitory Factors MIF 1 and MIF 2 cDNAAiti Vizzini
21. Purification of Antibodies against Entamoeba histolytica MIF and their use in Analyzing Human and Mouse Samples Laura Farr, Koji Watanabe, and Shannon Moonah
22. Studying Plant MIF/D-DT-Like Genes and Proteins (MDLs) Dzmitry Sinitski, Katrin Gruner, Jurgen Bernhagen, and Ralph Panstruga