Main description:

This volume details methods and protocols covering multiple aspects of Medulloblastoma. Divided into four parts, chapters guide readers through nucleic acids detection and analysis, cell-based analysis methodologies, and applications of patient-information on designing better experimental strategies for future drug development efforts in Medulloblastoma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Medulloblastoma: Methods and Protocols aims to deliver a clear-cut and standardized set of protocols to a broad scientific community.

Contents:

1. Childhood Medulloblastoma: An Overview

Yujin Suk, William D Gwynne, Ian Burns, Chitra Venugopal and Sheila K Singh

Part I: Molecular Assay-based Methods

2. Real-time PCR assays on formalin-fixed paraffin embedded medulloblastomas

Ratika Kunder and Neelam Vishwanath Shirsat

3. Validation of microRNA target genes using luciferase reporter assay and western blot analysis

Pooja Panwalkar, Atul Khire and Neelam Shirsat

4. Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY

Tara Dobson and Jyothishmathi Swaminathan

5. Quantification of microRNAs in cells and tissues using stem-loop RT PCR and qPCR

Pooja Panwalkar, Atul Khire and Neelam Shirsat

6. Analysis of Microarray Data from Medulloblastoma tissue samples

Debojyoti Dhar and Gopala Kallapura

7. Advancement of single- cell sequencing in Medulloblastoma

Deepanshu Verma, Namyashree Nayak, Ashuthosh Singh, Ashutosh Kumar Singh, Neha Garg

Part II: Cellular Assays and Inhibitors for Medulloblastoma

8. Flow cytometry approaches to obtain medulloblastoma stem cells from bulk cultures

Praveen Kumar and Neha Garg

9. Drug Screening assays on Medulloblastoma Stem Cells using compound libraries

Ashutosh Singh and Neha Garg

10. 3D-on-2D: A physiologically-relevant and gel-free in vitro co-culture method to assay anti-metastatic agents

Abhinav Dey, Samuel Eisenberg, Rayna Birnbaum and David J Sharp

11. In vitro screening assay for activators of T- cell migration to solid tumors

Abhinav Dey and David Sharp

12. Impact of Nano preparation of phytoconstituents in Medulloblastoma

Priyanka Mishra and Y.B. Tripathi

13. Challenges and Recent Advances of novel chemical inhibitors in Medulloblastoma Therapy

Anand Maurya, Upendra Kumar Patel, Jitendra Kumar Yadav, Virender Pratap Singh, Alka Agarwal

14. Role of ayurvedic plants as anti-cancer agents

Nikhil Pandey and Y.B. Tripathi

Part III: Animal Models in Medulloblastoma

15. In vivo tumorigenicity assays using subcutaneous and orthotopic xenografts in immunodeficient mice

Kedar Yogi, Rahul Thorat and Neelam Shirsat

16. Manufacture of an Oncolytic Measles Virus-Sensitive Immune-Competent Mouse Model of Medulloblastoma.

Sangeet Lal and Corey Raffel

Part IV: Patient data based approaches for Biomedical Research in Medulloblastoma

17. Preclinical Research Strategy Development for RNAi-based therapies in Oncology using patient-centred information from public databases of Human Protein Atlas and R2Genomics Platform

Abhinav Dey, Isabelle Balachandran, Ava Solis and David J Sharp

18. Magnetic resonance imaging in the contemporary management of medulloblastoma: current and emerging applications

Archya Dasgupta, Madan Maitre, Sona Pungavkar and Tejpal Gupta

19. Multidisciplinary Management of Medulloblastoma: Consensus, Challenges and Controversies

Abhishek Chatterjee, Madan Maitre, Archya Dasgupta, Epari Sridhar and Tejpal Gupta