Main description:

This volume discusses the latest analytical approaches used to sample defined molecular populations of metabolites via functional group derivatization, specialized chromatographic methods, and ionization techniques. Chapters cover key methods for sample introductions to the ion source, including direct flow, gas chromatography, liquid chromatography, and capillary electrophoresis. Chapters also explore non-targeted and targeted analyses, as well as the emerging field of metallomics. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Metabolomics is a valuable resource for students, researchers, practicing physicians and veterinarians, and administrators involved in the funding of research.

Contents:

Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1. Flow Infusion ESI High Resolution Mass Spectrometry Metabolomics Analytical Platforms
Paul L. Wood

2. High Throughput Metabolomics using Flow Injection Analysis and Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry
Estelle Rathahao-Paris, Sandra Alves, and Alain Paris

3. Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics
Miriam C. Perez-Cova, Roma Tauler, and Joaquim Jaumot

4. Metabolomics of Small Numbers of Cells using Chemical Isotope Labeling Combined with Nanoflow LC-MS
Xian Luo and Liang Li

5. Untargeted Metabolomics to Interpret the Effects of Social Defeat: Integrating Chemistry and Behavioral Approaches in Neuroscience
Allen K. Bourdon and Brooke N. Dulka

6. Flow Infusion Electrospray Ionization High Resolution Mass Spectrometry of the Chloride Adducts of Sphingolipids, Glycerophospholipids, Glycerols, Hydroxy Fatty Acids, Fatty Acid Esters of Hydroxy Fatty Acids (FAHFA), and Modified Nucleosides
Paul L. Wood and Randall L. Woltjer

7. High Mass Accuracy Gas Chromatography/Mass Spectrometry for Performing Isotopic Ratio Outlier Analysis: Applications for Non-Annotated Metabolite Detection
Yunping Qiu and Irwin J. Kurland

8. Enantioseparation and Detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by Chiral Gas Chromatography-Triple Quadrupole Mass Spectrometry
Shinji K. Strain, Morris D. Groves, and Mark R. Emmett

9. Enantioselective Supercritical Fluid Chromatography (SFC) for Chiral Metabolomics
Robert Hofstetter, Andreas Link, and Georg M. Fassauer

10. Capillary Electrophoresis-Mass Spectrometry of Hydrophilic Metabolomics
Masahiro Sugimoto

11. Lipidomic Analysis of Oxygenated Polyunsaturated Fatty Acid-Derived-Inflammatory Mediators in Neurodegenerative Diseases
Mauricio Mastrogiovannim Estefania Ifran, Andres Trostchansky, and Homero Rubbo

12. The Characterization and Quantification of the Fatty Acid Amidome
Kristen A. Jeffries, Emma K. Farrell, Ryan L. Anderson, Gabriela Suarez, Amanda J. Goyette Osborne, McKenzi K. Heide, and David J. Merkler

13. Metabolomics Analysis of Complex Biological Specimens using Nuclear Magnetic Resonance Spectroscopy
Khushboo Gulati, Sharanya Sarkar, and Krishna Mohan Poluri

14. Untargeted Metabolomics Methods to Analyze Blood Derived Samples
Danuta Dudzik and Antonia Garcia

15. Multi-Compartmental High-Throughput Metabolomics Based on Mass Spectrometry
Raul Gonzalez-Dominguez, Alvaro Gonzalez-Dominguez, Ana Sayago, and Angeles Fernandez-Recamales

16. GC-MS Non-Targeted Metabolomics of Neural Tissue
Carolina Gonzalez-Riano, M Fernanda Rey-Stolle, and Antonia Garcia

17. GC-MS of Pentafluourobenzyl Derivatives of Phenols, Amines, Carboxylic Acids
Paul L. Wood

18. GC-MS of tert-Butyldimethylsilyl (tBDMS) Derivatives of Neurochemicals
Paul L. Wood

19. UHPLC-MS/MS Method for Determination of Biologically Important Thiols in Plasma using New Derivatizing Maleimide Reagent
Dominika Olesova and Andrej Kovac

20. Untargeted Metabolomics Determination of Postmortem Changes in Brain Tissue Samples by UHPLC-ESI-QTOF-MS and GC-EI-Q-MS
Carolina Gonzalez-Riano, Antonia Garcia, and Coral Barbas

21. Metallomics Imaging
Valderi Luiz Dressler, Graciela Marini Hiedrich, Vinicius Machado Neves, Eson Irineu Muller, and Dirce Pozebon

Subject Index List...