Main description:

Versatile, comprehensive, and clearly written, this competitively priced laboratory manual can be used with any undergraduate microbiology text-and now features brief clinical applications for each experiment, and a new experiment on hand washing. Microbiology: A Laboratory Manual is known for its thorough coverage, descriptive and straightforward procedures, and minimal equipment requirements. A broad range of experiments helps to convey basic principles and techniques. Each experiment includes an overview, an in-depth discussion of the principle involved, easy-to-follow procedures, and lab reports with review and critical thinking questions. Ample introductory material and laboratory safety instructions are provided.

Contents:

Part 1: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms 1. Effectiveness of Hand Washing 2. Culture Transfer Techniques 3. Techniques for Isolation of Pure Cultures 4. Cultural Characteristics of Microorganisms Part 2: Microscopy 5. Microscopic Examination of Stained Cell Preparations 6. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount 7. The Microscopic Measurement of Microorganisms Part 3: Bacterial Staining 8. Preparation of Bacterial Smears 9. Simple Staining 10. Negative Staining 11. Gram Stain 12. Acid-Fast Stain 13. Differential Staining for Visualization of Bacterial Cell Structures Part 4: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations 14. Nutritional Requirements: Media for the Routine Cultivation of Bacteria 15. Use of Differential, Selective, and Enriched Media 16. Physical Factors: Temperature 17. Physical Factors: pH of the Extracellular Environment 18. Physical Factors: Atmospheric Oxygen Requirements 19. Techniques for the Cultivation of Anaerobic Microorganisms 20. Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells 21. The Bacterial Growth Curve Part 5: Biochemical Activities of Microorganisms 22. Extracellular Enzymatic Activities of Microorganisms 23. Carbohydrate Fermentation 24. Triple Sugar-Iron Agar Test 25. IMViC Test 26. Hydrogen Sulfide Test 27. Urease Test 28. Litmus Milk Reactions 29. Nitrate Reduction Test 30. Catalase Test 31. Oxidase Test 32. Utilization of Amino Acids 33. Genus Identification of Unknown Bacterial Cultures Part 6: The Protozoa 34. Free-Living Protozoa 35. Parasitic Protozoa Part 7: The Fungi 36. Cultivation and Morphology of Molds 37. Yeast Morphology, Cultural Characteristics, and Reproduction 38. Identification of Unknown Fungi Part 8: The Viruses 39. Cultivation and Enumeration of Bacteriophages 40. Isolation of Coliphages from Raw Sewage Part 9: Physical and Chemical Agents for the Control of Microbial Growth 41. Physical Agents of Control: Moist Heat 42. Physical Agents of Control: Environmental Osmotic Pressure 43. Physical Agents of Control: Electromagnetic Radiations 44. Chemical Agents of Control: Chemotherapeutic Agents 45. Determination of Penicillin Activity in the Presence and Absence of Penicillinase 46. Chemical Agents of Control: Disinfectants and Antiseptics Part 10: Microbiology of Food 47. Microbiological Analysis of Food Products: Bacterial Count 48. Wine Production Part 11: Microbiology of Water 49. Standard Qualitative Analysis of Water 50. Quantitative Analysis of Water: Membrane Filter Method Part 12: Microbiology of Soil 51. Microbial Populations in Soil: Enumeration 52. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates 53. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique Part 13: Bacterial Genetics 53. Enzyme Induction 54. Bacterial Conjugation 55. Isolation of a Streptomycin-Resistant Mutant 56. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity Part 14: Biotechnology 57. Bacterial Transformation 58. Isolation of Bacterial Plasmids 59. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA Part 15: Medical Microbiology 60. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries 61. Normal Microbial Flora of the Throat and Skin 62. Identification of Human Staphylococcal Pathogens 63. Identification of Human Streptococcal Pathogens 64. Identification of Streptococcus pneumoniae 65. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems 66. Isolation and Presumptive Identification of Campylobacter 67. Microbiological Analysis of Urine Specimens 68. Microbiological Analysis of Blood Specimens 69. Species Identification of Unknown Bacterial Cultures Part 16: Immunology 70. Precipitin Reaction: The Ring Test 71. Agglutination Reaction: The Febrile Antibody 72. Immunofluorescence 73. Enzyme-Linked Immunoabsorbent Assay 74. Agglutination Reaction: Mono-Test for Infectious Mononucleosis 75. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures Appendices