Main description:

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and "omics" analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.

Contents:

Part I: Microglia in Health and Disease

1. Microglia: The Neural Cells of Non-Neural Origin

Olga Garaschuk and Alexei Verkhratsky

2. Morphology of Microglia across Contexts of Health and Disease

Julie C. Savage, Micael Carrier, and Marie-Eve Tremblay

3. Physiology of Microglia

Olga Garaschuk and Alexei Verkhratsky

4. Microglia Reactivity: Heterogeneous Pathological Phenotypes

Helene Hirbec, Francois Rassendren, and Etienne Audinat

5. Microglia in Neurodegenerative Disorders

Dario Tejera and Michael T. Heneka

6. Physiological Implications of Microglia-Synapse Interactions

Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, and Junichi Nabekura

Part II: In Vitro Analyses of Microglia

7. Ultrastructural Analyses of Microglial Interactions with Synapses

Marie-Eve Tremblay and Ania K. Majewska

8. Visualizing Dark Microglia

Marie-Kim St-Pierre, Maude Bordeleau, and Marie-Eve Tremblay

9. Electrophysiological Investigation of Microglia

Elena Avignone, Giampaolo Milior, Isabelle Arnoux, and Etienne Audinat

10. Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia

Annette Masuch and Knut Biber

11. Assessment of Parasite-Microglia Interactions In Vitro

Katherine Figarella, Nestor L. Uzcategui, and Michael Duszenko

Part III: Studying Microglia In Vivo

12. In Vivo Visualization of Microglia Using Tomato Lectin

Bianca Brawek, Maria Olmedillas del Moral, and Olga Garaschuk

13. Long-Term In Vivo Imaging of Individual Microglial Cells

Angelos A. Skodras, Jasmin Hefendehl, and Jonas J. Neher

14. Analysis of Signaling Mechanisms Regulating Microglial Process Movement

Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, and Christian Madry

15. Measuring Microglial Turnover in the Adult Brain

Diego Gomez-Nicola, Gemma L. Fryatt, and Katharine E. Askew

16. Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor

Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, and Ari Waisman

17. Single Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia

Bianca Brawek and Olga Garaschuk

18. Labeling Microglia with Genetically-Encoded Calcium Indicators

Yajie Liang and Olga Garaschuk

19. Calcium Imaging of Microglial Network Activity in Stroke

Petr Tvrdik, Kathryn N. Kearns, Khadijeh A. Sharifi, M. Filip Sluzewski, Scott T. Acton, and M. Yashar S. Kalani

20. In Vivo Two Photon Imaging of Microglial Synapse Contacts

Daisuke Kato, Ako Ikegami, Hiroshi Horiuchi, Andrew J. Moorhouse, Junichi Nabekura, and Hiroaki Wake

21. In Vivo Phagocytosis Analysis of Amyloid Beta

Dario Tejera and Michael T. Heneka

22. Functional Analysis of Brain-Engrafted Monocytes after Microglial Ablation in Mouse Models

Nicholas H. Varvel, Richard M. Ransohoff, and Jonas J. Neher

Part IV: Omics Analyses of Microglia

23. Analysis of the Microglial Sensome

Suzanne E. Hickman and Joseph El Khoury

24. Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue

Giampaolo Milior, Farah Chali, Tiffany Dos Santos, Juliette Royer, Richard Miles, and Melanie Morin-Brureau