Main description:

This detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.

Contents:

Part I: NK Cell Specification

1. Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria

Ao Mei, Elaheh Hashemi, Mohamed Khalil, Dandan Wang, and Subramaniam Malarkannan

2. Purification of Primary Decidual Natural Killer Cells for Functional Analysis

Angela C. Crespo, Aria Alexander, and Tamara Tilburgs

3. In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells

Ines Ullmo, Nahide Koksal, Heather Y.K. Ang, and Hugh J.M. Brady

4. Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells

Akira Niwa and Megumu K. Saito

5. Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease

Liang Shan, Richard A. Flavell, and Dietmar Herndler-Brandstetter

6. Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics' Single-Cell RNA-Seq

Dominic Paul Lee, Wang Jiehao Ray, Tan Pee Mei, Shawn Hoon, Jonathan Scolnick, and Gene W. Yeo

7. Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses

Dandan Wang, Robert Burns, Mohamed Khalil, Ao Mei, Elaheh Hashemi, and Subramaniam Malarkannan

8. Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells

Elaheh Hashemi, Ao Mei, Dandan Wang, Mohamed Khalil, and Subramaniam Malarkannan

9. Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes

Simon Grassmann, Joseph C. Sun, and Veit R. Buchholz

10. Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis

Amera L. Martinez, Michael J. Shannon, Shira E. Eisman, Everardo Hegewisch-Solloa, Aneeza N. Asif, Tasneem A.M. Ebrahim, and Emily M. Mace

11. Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling

Noriko Sato, Lawrence P. Szajek, and Peter L. Choyke

Part II: NK Cell Functional Assays

12. Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer

Javier Traba and Olga M. Anton

13. Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors

Lizeth G. Meza Guzman and Sandra E. Nicholson

14. Method to Study Adaptive NK Cells Following MCMV Infections

Mohamed Khalil, Ao Mei, Elaheh Hashemi, Dandan Wang, Megan Schumacher, Scott Terhune, and Subramaniam Malarkannan

15. Assessing the Response of Human NK Cell Subsets to Infection by Clinically-Isolated Virus Strains

Nobuyo Yawata and Makoto Yawata

16. NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA -EuTDA Cytotoxicity Assay

Casey W. Buller and Stephen O. Mathew

17. Organoid Co-Culture Methods to Capture Cancer Cell -Natural Killer Cell Interactions

Isaac S. Chan and Andrew J. Ewald

18. In Vitro Visualization of Cell-to-Cell Interactions between Natural Killer Cells and Sensory Neurons

Hyoung Woo Kim, Alexander J. Davies, and Seog Bae Oh

19. CRISPR Screen to Identify Factors that Render Tumor Cells Sensitive or Resistant to Killing by NK Cells

Xiaoxuan Zhuang and Eric O. Long

Part III: Clinical Application

20. Practical Considerations and Workflow in Utilizing KIR Genotyping in Transplantation Medicine

Makoto Yawata and Nobuyo Yawata

21. Gene Transduction of Natural Killer Cells for Clinical Application

Noriko Shimasaki