Main description:

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.

Contents:

Part I: Introductory Reviews

1. Neural Transplantation and Stem Cells
Mahendra S. Rao and Mohan C. Vemuri

2. Adult Stem Cells for the Treatment of Neurological Disease
C. M. Rice and Neil J. Scolding

3. Human Trials for Neurodegenerative Disease
Claire M. Kelly, O. J. Handley, and A.E. Rosser

Part II: Practical/Methodological Chapters

4. Differentiation of Neuroepithelia from Human Embryonic Stem Cells
Xiaofeng Xia and Su-Chun Zhang

5. Derivation of High Purity Oligodendroglial Progenitors
Maya N. Hatch, Gabriel Nistor, and Hans S. Keirstead

6. Flow Cytometric Characterization of Neural Precursor Cells and their Progeny
Preethi Eldi and Rodney L. Rietze

7. Isolation, Expansion and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using the Neurosphere Assay
Loic P. Deleyrolle and Brent A. Reynolds

8. Human Mesenchymal Stem Cell Culture for Neural Transplantation
David Gordon and Neil J. Scolding

9. Umbilical Cord Blood Cells
Jennifer D. Newcomb, Alison E. Willing and Paul R. Sanberg

10. Animal Models of Neurodegenerative Diseases
Wendy Phillips, Andrew Michell, Harald Pruess, and Roger A. Barker

11. Animal Models of Multiple Sclerosis
Roberto Furlan, Carmela Cuomo, and Gianvito Martino

12. Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies
Yoichi Kondo and Ian D. Duncan

13. A Rat Middle Cerebral Artery Occlusion (MCAO) Model and Intravenous Cellular Delivery
Masanori Sasaki, Osamu Honmou, and Jeffery D. Kocsis

14. MR Tracking of Stem Cells in Living Recipients
Eva Sykova, Pavla Jendelova, and Vit Herynek

15. Identifying Neural Progenitor Cells in the AdultBrain
Stephen Kelly, Maeve Caldwell, Matthew P. Keasey, Jessica A. Cooke, and James B. Uney

16. Immune Ablation Followed by Autologous Hematopoietic Stem Cell Transplantation for the Treatment of Poor Prognosis Multiple Sclerosis
Harold Atkins and Mark Freedman