Main description:

This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.

Contents:

1. Culturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition

Hassan Azari

2. Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region

Alessandra Ricca, Federica Cascino, and Angela Gritti

3. Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres

Ruslan Rust and Tara L. Walker

4. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord

Jean-Philippe Hugnot

5. Culturing and Expansion of "Clinical Grade" Neural Stem Cells from the Fetal Human Central Nervous System

Maurizio Gelati, Daniela Celeste Profico, Daniela Ferrari, and Angelo Luigi Vescovi

6. Microglia Isolation from Neural Stem Cell-Enriched Regions

Gregory P. Marshall II

7. Microglial FACS Sorting for Robust RNA Recovery for Next Generation Sequencing

Andria L. Doty and Aline Oliveria

8. Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded under Neurosphere Culture

Nasser Nassiri Koopaei, Thomas D. Schmittgen, Brent A. Reynolds, and Hassan Azari

9. Isolating and Culturing of Precursor Cells from the Adult Human Brain

Florian A. Siebzehnrubl

10. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord

Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahaye, Alain Boularan, Alain Privat, and Jean-Philippe Hugnot

11. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny

Hassan Azari

12. Identifying Neural Progenitor Cells in the Adult Human Brain

Thomas I.H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L.M. Faull, Mike Dragunow, and Maurice A. Curtis

13. Neonatal Transplant in Hypoxic Injury

Tong Zheng and Michael D. Weiss

14. Isolation and Purification of Self Renewable Human Neural Stem Cells from iPSCs for Cell Therapy in Experimental Model of Ischemic Stroke

Marcel M. Daadi

15. Generating Cerebral Organoids from Human Pluripotent Stem Cells

Leon Chew, Adam Anonuevo, and Erin Knock

16. Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue

Maurice Curtis and Vinata Vedam-Mai

17. Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases

Vinata Vedam-Mai