Main description:

This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.

Contents:

Part I: Saliva and Other Oral Fluids

1. RNA Sequencing Analysis of Saliva exRNA

Karolina Elzbieta Kaczor-Urbanowicz and David T.W. Wong

2. Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry

Nagihan Bostanci and Kai Bao

3. Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics

Pingping Han, Xiang Li, Wei Wei, and Saso Ivanovski

4. Antioxidant Micronutrients and Oxidative Stress Biomarkers

Irundika H.K. Dias, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, Iain L.C. Chapple, and Melissa M. Grant

Part II: Molecular Biosciences

5. The Oral Microbiota in Health and Disease: An Overview of Molecular Findings

Jose F. Siqueira Jr and Isabela N. Rocas

6. The Long and Short of Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes

Nicholas C.K. Heng and Jo-Ann L. Stanton

7. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

Jose F. Siqueira Jr, Mitsuo Sakamoto, and Alexandre S. Rosado

8. Bioinformatic Approaches for Describing the Oral Microbiota

Kristi Biswas, Michael W. Taylor, and David T.J. Broderick

9. Adhesion of Yeast and Bacteria to Oral Surfaces

Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, and Ann R. Holmes

10. Quantitative Analysis of Periodontal Pathogens Using Real Time Polymerase Chain Reaction (PCR)

M Jose Marin, Elena Figuero, David Herrera, and Mariano Sanz

11. Methods to Study Antagonistic Activities Among Oral Bacteria

Fengxia Qi and Jens Kreth

12. Generation of Multi-Species Oral Bacteria Biofilm Models

Jason L. Brown, Mark C. Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston, and Gordon Ramage

13. Markerless Genome Editing in Competent Streptococci

Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. Amdal, Fernanda C. Petersen, and Donald A. Morrison

14. A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System

Sven E. Niklander and Keith D. Hunter

15. Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients

Sai Shyam Vasantharajan, Edward Barnett, Elin S. Gray, Euan J. Rodger, Michael R. Eccles, Sharon Pattison, Fran Munro, and Aniruddha Chatterjee

16. Strategy for RNA-Seq Experimental Design and Data Analysis

Gregory Gimenez, Peter A. Stockwell, Euan J. Rodger, and Aniruddha Chatterjee

17. Characterization of the Expression and Role of Histone Acetylation and Deacetylation in Dental Pulp Cells

Yukako Yamauchi and Henry F. Duncan

18. Genome-Wide Analysis of Periodontal and Peri-Implant Cells and Tissues

Moritz Kebschull, Annika Therese Kroeger, and Panos N. Papapanou

19. Differential Expression, Functional and Machine Learning Analysis of High-Throughput -Omics Data Using Open-Source Tools

Moritz Kebschull, Annika Therese Kroeger, and Panos N. Papapanou

20. Micro-RNA Profiling in Dental Pulp Cell Cultures

Michaela Kearney and Henry F. Duncan

Part III: Cells and Tissues

21. Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease

Mike R. Milward, Martin R. Ling, Melissa M. Grant, Joanna Batt, and Iain L.C. Chapple

22. A Cell Culture Method for the Isolation and Study of Primary Human Dental Pulp Cells

Shelly Arora, Benedict Seo, Lara Friedlander, and Haizal Mohd Hussaini

23. Culturing Adipose-Derived Stem Cells Under Serum-Free Conditions

Diogo Godoy Zanicotti, Trudy J. Milne, and Dawn E. Coates

24. Quantitative Real-Time Gene Profiling of Human Alveolar Osteoblasts Using a One-Step System

Dawn E. Coates, Sobia Zafar, and Trudy J. Milne

25. Fabrication and Characterization of Decellularized Periodontal Ligament Cell Sheet Constructs

Amro Farag, Cedryck Vaquette, Dietmar W. Hutmacher, Mark Bartold, and Saso Ivanovski

26. Immunohistochemistry and Immunofluorescence

Haizal Mohd Hussaini, Benedict Seo, and Alison M. Rich

27. Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Josefine Hirschfeld, Ilaria J. Chicca, Carolyn G.J. Moonen, Phillipa C. White, Martin R. Ling, Helen J. Wright, Paul R. Cooper, Mike R. Milward, and Iain L.C. Chapple

28. Cell Seeding on 3D Scaffolds for Tissue Engineering and Disease Modeling Applications

Fanny Blaudez, Cedryck Vaquette, and Saso Ivanovski

29. Workflow for Fabricating 3D-Printed Resorbable Personalized Porous Scaffolds for Orofacial Bone Regeneration

Cedryck Vaquette, Danilo Carluccio, Martin Batstone, and Saso Ivanovski

30. Methacrylated Gelatin as an On-Demand Injectable Vehicle for Drug Delivery in Dentistry

W. Benton Swanson, Abdul Hameed Mahmoud, Seth Woodbury, and Marco C. Bottino

31. In Vitro Biological Testing of Dental Materials

Jithendra Ratnayake, Josette Camilleri, T. Nethmini Haththotuwa, and Jeffrey Huang