Main description:

This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ovarian Cancer: Methods and Protocols serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find new therapeutic approaches.

Contents:

Part I: Clinical Perspectives

1. Clinical Staging of Ovarian Cancer

Andrea S. O'Shea

2. Pathologic Classification of Ovarian Cancer

Stephanie M. McGregor

Part II: Tumor Microenvironment

3. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer

Jessica Vazquez, Dagna Sheerar, Aleksandar K. Stanic, and Manish S. Patankar

4. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors

Veronica D. Gonzalez, Ying-Wen Huang, and Wendy J. Fantl

5. Processing and Analysis of Ascites

Hannah M. Micek, Molly J. Carroll, Lisa Barroilhet, and Pamela K. Kreeger

6. Multispectral Staining and Analysis of Extracellular Matrix

Carine M. Renner, Mike R. Visetsouk, Pamela K. Kreeger, and Kristyn S. Masters

7. Quantitative Analysis of the Extracellular Matrix by Immunoblot

Kaitlin C. Fogg

Part III: Cell Isolation

8. Multiparameter Single Cell Characterization of Ovarian Intratumor Heterogeneity

Kristin G. Beaumont, Christina Andreou, Ethan Ellis, and Robert Sebra

9. Culturing Primary Human Mesothelial Cells

Mary Mullen, Hollie Noia, and Katherine Fuh

10. Isolation of Normal and Cancer-Associated Fibroblasts

Katarzyna Zawieracz and Mark A. Eckert

11. Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum

Abir Mukherjee

Part IV: Model Systems

12. Isolation of Fallopian Tube Epithelium and Ovaries for Assessment of Cilia Beating Frequency (CBF)

Angela Russo and Joanna E. Burdette

13. Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer

Matthew Dean

14. In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer

Elizabeth I. Harper and Tyvette S. Hilliard

15. The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance: 3D Co-Culture Methods

Michael Bregenzer, Eric Horst, Pooja Mehta, Catherine Snyder, Taylor Repetto, and Geeta Mehta

16. Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection

Sonam Mittal, Prachi Gupta, Pradeep Chaluvally-Raghavan, and Sunila Pradeep

17. Humanized Patient-Derived Xenograft Models of Ovarian Cancer

Sarah B. Gitto, Erin George, Sergey Medvedev, Fiona Simpkins, and Daniel J. Powell, Jr.

18. Xenograft Models of Ovarian Cancer for Therapy Evaluation

Mihaela Popa, Vibeke Fosse, Katrin Kleinmanns, Line Bjorge, and Emmet McCormack

19. Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer

Dylan O. Matvey, Thomas S.C. Ng, and Miles A. Miller