Main description:

This detailed volume provides an updated overview of current uses of peptide microarray technology, showcasing consolidated applications while highlighting some of the most intriguing novelties and emerging fields of use. The methodologies within this collection are of considerable value for both advanced users and new-comers in the peptide microarray arena, as the renowned contributors describe full coverage of the aspects related to their workflows, from microchip manufacturing to advanced analytical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Peptide Microarrays: Methods and Protocols, Third Edition aims to make the use of peptide microarrays more and more accessible while stimulating further developments to sustain exciting discoveries in the biochemistry and medicine realms.

Contents:

1. Applications of Peptide Microarrays in Autoantibody, Infection, and Cancer Detection

Carsten Groetzinger

2. Peptide Microarrays for Studying Autoantibodies in Neurological Disease

Ivan Talucci and Hans Michael Maric

3. A Bi-Functional Polymeric Coating for the Co-Immobilization of Proteins and Peptides on Microarrays Substrates

Laura Sola, Dario Brambilla, Alessandro Mussida, Francesco Damin, and Marcella Chiari

4. Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry

Denis Prim, Milica Jovic, and Marc E. Pfeifer

5. Hybrid Peptide-Agarose Hydrogels for 3D Immunoassays

Angelo Musico, Greta Bergamaschi, Alessandro Strada, Roberto Frigerio, Paola Gagni, Marina Cretich, and Alessandro Gori

6. Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes

Kenna Nagy, Ryan McBride, Steven R. Head, Phillip Ordoukhanian, and Mansun Law

7. Fast Protocols for Characterizing Antibody-Peptide Binding

Sophie Cleaver, Matthew Gardner, Anthony Barlow, Enrico Ferrari, and Mikhail Soloviev

8. Peptides and Anti-Peptide Antibodies for Small and Medium Scale Peptide and Anti-Peptide Affinity Microarrays

Fan Zhang, Sophie Cleaver, Matthew Gardner, Andrea Briones, and Mikhail Soloviev

9. One-Shot Generation of Epitope-Directed Monoclonal Antibodies to Multiple Non-Overlapping Targets: Peptide Selection, Antigen Preparation, and Epitope Mapping

Oi Wah Liew, Samantha Shi Min Ling, Shera Lilyanna, Jenny Pek Ching Chong, Jessica Yan Xia Ng, and Arthur Mark Richards

10. Peptide Microarray-Based Protein Interaction Studies Across Affinity Ranges: Enzyme Stalling, Cross-Linking, Depletion, and Neutralization

Clemens Schulte, Vladimir Khayenko, and Hans Michael Maric

11. Profiling of Multiple Matrix Metalloproteinases Activities in the Progression of Osteosarcoma by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Coated Zinc Oxide Nanorod Substrate

Zhenxin Wang, Minghong Jian, and Xiaotong Li

12. Peptide Array-Based In Situ Fluorescence Assay for Profiling Multiple Matrix Metalloproteinases Activities

Zhen Lei and Zhenxin Wang

13. Peptide-Based Sensor and Microfluidic Platform for IgG Antibody Detection by Differential Impedance Sensing

Paola Piedimonte, Laura Sola, Marcella Chiari, Giorgio Ferrari, and Marco Sampietro

14. Peptide Microarrays for Flavivirus Diagnosis

Stella Garcia Colombarolli, Izabella Cristina Andrade Batista, Naiara Clemente Tavares, Eneida Santos de Oliveira, Camila Sales Nascimento, Philip Louis Felgner, Rafael Ramiro de Assis, and Carlos Eduardo Calzavara-Silva

15. Epitope Mapping on Microarrays, Highlight a Sequence on the N Protein with Strong Immune-Response in SARS-CoV-2 Patients

Roberto Frigerio, Angelo Musico, Alessandro Strada, Alessandro Mussida, Paola Gagni, Greta Bergamaschi, Marcella Chiari, Luisa Barzon, Alessandro Gori, and Marina Cretich

16. IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay

Javier Martinez-Botas, Carlos Fernandez-Lozano, Aida Vaquero-Rey, and Belen de la Hoz

17. Extracellular Vesicles Molecular Profiling for Diagnostic Purposes: An Application of Phage-Display Technology

Stella Garcia Colombarolli, Alberto Vitali, and Francesca Sciandra

18. Membrane-Sensing Peptides for Extracellular Vesicles Analysis

Alessandro Strada, Roberto Frigerio, Greta Bergamaschi, Paola Gagni, Marina Cretich, and Alessandro Gori